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《Journal of Clinical Otorhinolaryngology Head and Neck Surgery》 2011-04
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Comparison of lentivirus and adenovirus vector mediated gene transfer into cultured spiral ganglion cells

WANG Guopeng1 LUO Linghui2 XIE Jing1 CHEN Pei2 FU Yong3 ZHONG Gang2 GONG Shusheng1(1Department of Otolaryngology Head and Neck Surgery,Beijing Tongren Hospital,Capital Medical University,Beijing,100730,China;2Department of Otolaryngology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology;3Department of Otolaryngology,the First Affiliated Hospital,College of Medicine,Zhejiang University)  
Objective:We aimed to compare the characteristics between lentivirus and adenovirus vector mediated gene transfer into cultured spiral ganglion cells(SGCs).Method:SGCs from newborn rats were cultured and exposed to lentivirus-GFP and adenovirus-GFP vectors.GFP expression and the cell morphology were evaluated under epi-fluorescence microscope at 3 days and 7 days after exposure.Survival number of SGCs was counted,and the average percentage of SGCs with GFP expression was calculated,and axon length was measured by ImageJ software.Result:Cultured SGCs were transfected by either adenovirus or lentivirus vector successfully.The adenovirus vector presented an instant and efficient transfection.However,the expression of GFP went down after 7 days.In lentivirus-GFP group,GFP expression was detected at 7 days after exposure,and the number of cells with GFP expression increased gradually in the following days.Statistical analysis revealed that there were no differences in survival number of SGCs and average axon length among lentivirus-GFP group,adenovirus-GFP group and control group.Conclusion:Cultured SGCs can be transfected by either lentivirus vector or adenovirus vector safely and efficiently.SGCs are more susceptible to adenovirus vector,but GFP persists for a longer period after the lentivirus-mediated gene transfer.
【Fund】: 国家自然科学基金(No:30872858);; 北京市自然科学基金(No:7082024);; 北京市教育委员会科技发展计划项目(No:KM200810025004)
【CateGory Index】: R764.43
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 LUO Ling-hui,GONG Shu-sheng, SONG Peng, YAN Kai-sheng,LIU Ying-peng. Department of Otorhinolaryngology, Union Hospital of Tongji Medical College,Huazhong University of Science and Technology, Wuhan 430022,China;Construction of recombinant adenovirus containing human Bcl-2 and its expression in the spiral ganglion cells[J];Chinese Journal of Otorhinolaryngology;2005-07
【Co-citations】
Chinese Journal Full-text Database 3 Hits
1 LUO Linghui Department of Otolaryngology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,Hubei,430022,China GONG Shusheng Department of Otolaryngology Head and Neck Surgery,Beijing Tongren Hospital,Capital Medical University,Key Laboratory of Otolaryngology Head and Neck Surgery(Capital Medical University),Ministry of Education,Beijing,100730,China;Adenovirus-mediated gene transfer of Bcl-2 promote the survival of primary culture of spiral ganglion cells in vitro[J];Chinese Archives of Otolaryngology-Head and Neck Surgery;2008-08
2 MA Chao, CHEN Yong, GONG Li, et al (Lanzhou Institute of Biological Products, Lanzhou 730046, China);Construction and Packaging of Recombinant Adenovirus Vector Carrying gB Gene of Human Cytomegalovirus[J];Chinese Journal of Biologicals;2008-07
3 LUO Ling-hui,GONG Shu-sheng,CHEN Pei(Department of Otolaryngology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China);Study of the protection of adenovirus-mediated gene transfer of Bcl-2 to the primary cultured spiral ganglion cells agaist apoptosis induced by oxidative stress[J];Chinese Journal of Otorhinolaryngology-Skull Base Surgery;2008-03
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