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《Journal of Clinical Otorhinolaryngology Head and Neck Surgery》 2011-04
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L-NAC protect hair cells in the rat cochlea from injury of exposure to styrene

YANG Weiping1 HU Bohua2 CHEN Guangdi2 Eric C BIELEFELD2 Donald HENDERSON2(1Institute of Otolaryngology,Chinese General Hospital of PLA,Beijing,100853,China;2Center for Hearing and Deafness,State University of New York,137 Cary Hall,3435 Main Street,Buffalo,NY 14214,USA)  
Objective:To observe the effects of N-acetyl-L-cysteine(L-NAC) protect hair cells in the rat cochlea from injury of exposure to styrene.Method:Seventeen adult Long Evans rats were used in present study.The animals were randomly assigned into test group(n=9) and control group(n=8).The animals were exposed to styrene by gavage at 400 mg/kg(2 g styrene was mixed with 1ml olive oil).Test group animals received styrene exposure plus L-NAC 325 mg/kg(L-NAC was dissolved in physiological saline solution) by intraperitoneal injection.Treatment was performed once a day,5 days per week for 3 weeks.Control group animals received the same volume of saline injection on an identical time schedule used for the test group.The auditory brainstem response(ABR) thresholds of both ears elicited with clicks were measured before and at the end of the 3-week styrene or styrene plus L-NAC treatment.After hearing was re-assessed,animals were sacrificed and cochleae were quickly removed from the skull.Following fixation,whole specimens comprising the basilar membrane with Corti's organ were separated from the modiolus.The organs of Corti were stained with propidium iodide(PI) and the TUNEL assay to visualize the morphologic viability of hair cell nuclei,FITC-labeled phalloidin,a F-actin intercalating fluorescent probe used to visualize the morphologic viability of cuticular plate and the stereocilia in the hair cells.Each organ of Corti was thoroughly examined using fluorescence microscopy.The numbers of damaged OHCs(apoptotic,necrotic and missing OHCs) were documented.Result:There was a statistically significant decrease in ABR threshold shift(P0.05)in the styrene-plus-L-NAC treated animals.The average percentage of damaged OHCs in the stytene-treated animals was 28.3%.In contrast,the average percentage of OHC damage in the styrene-plus-L-NAC treatmed group was only 10.6%(P0.01).The percentage of reduction in the number of apoptotic cells in styrene-plus-L-NAC treated group was 78%(P0.01).However,the mean reduction of necrotic cells was only 23%(P0.05).Conclusion:The results indicate that the treatment with L-NAC may effectively protect against the styrene-induced hair cells damage and preferably reduce the number of apoptotic OHCs.
【Fund】: 美国NIH资助课题(No:NIDCD P01-DC03600-1A1 NIDCD 1 R 01 OH008113-01A1)
【CateGory Index】: R764.3
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【Citations】
Chinese Journal Full-text Database 5 Hits
1 YANG Wei-ping,HU Bo-hua,Donald Henderson.Institute of Otolaryngology,General Hospital of PLA,Beijing 100853,China;Signal pathway associated with hair cell death in cochlea following exposure to intense impulse noise[J];Medical Journal of Chinese People's Liberation Army;2009-01
2 YANG Wei-ping, HU Bo-hua, Donald Henderson. Institute of Otolaryngology, General Hospital of PLA, Beijing 100853, China;The primary death pathway of outer hair cells of rat cochlea following styrene exposure[J];Medical Journal of Chinese People's Liberation Army;2009-07
3 YANG Weiping(Institute of Otolaryngology,General Hospital of PLA,Beijing,100853,China);Changes of filamentous-actin at different stages of apoptotic and necrotic hair cells in rat cochleas induced by noise exposure[J];Journal of Clinical Otorhinolaryngology Head and Neck Surgery;2010-13
4 SUN Jun, WANG Yu Xia, SUN Man Ji (Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China);N-acetyl L-cysteine and glutathione antagonize sulfur mustard in inducing cell apoptosis and necrosis[J];CHINESE JOURNAL OF PHARMACOLOGY AND TOXICOLOGY;2000-01
5 YANG Wei-ping1,HU Bo-hua21. Institute of Otolaryngology, PLA General Hospital, Beijing 100853, China2. Center for Hearing and Deafness, Department of Communicative Disorders and Sciences, State University of New York at Buffalo, 137 Cary Hall, 3435 Main Street, Buffalo, NY 14214, USA;Comparison of three methods for detecting the death modes of hair cells in the cochlea[J];;2004-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Hai Yun 1, CHANG Wen Jun 2, CHEN Zhi Long 1* , GU Chun Ying 2, ZHANG Li Ming 1, Lü Ting 1 (1. Department of Chemical Defense Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China; 2. Department of Epidemiology, Faculty of Health Service) [;Drugs in treating sulfur mustard-induced lesions and their mechanism[J];Academic Journal of Second Military Medical University;2003-06
2 LI Qi-hui1,DONG Zhao-jun2,SUN Hua-bin1,HUANG Wei-chu1,YANG Xin-fang1,WANG Xiu-de1,TANG Mu-tao1,SAI Yan2(1Center of Disease Prevention and Control,Jinan Military Command,Jinan 250014;2Department of Chemical Defense,College of Preventive Medicine,Third Military Medical University,Chongqing 400038,China);Reduced glutathione protects against DNA damage of CHL cells by sulfur mustard[J];Acta Academiae Medicinae Militaris Tertiae;2007-23
3 YU Jia-ming,XU Zhao-fa,WANG Jia-jun, XU Bin,YANG Jing-hua (School of Public Health,China Medical University,Shenyang 110001,China);Effect of Glutathione on Renal Cell Apoptosis Induced by Mercury[J];Industrial Health and Occupational Diseases;2005-05
4 ZHAO Su*,JIN Huan-rong*,ZHANG Fang(*Shenyang Medical College,Shenyang 110034 China);Effect of Styrene on Lipid Peroxidation in Blood and Liver Tissue in Rats[J];Industrial Health and Occupational Diseases;2007-04
5 Yang Weiping, Hu Bohua, Donald Henderson. Institute of Otolaryngology, General Hospital of PLA, Beijing 100853, China;Death mode of outer hair cells in chinchilla cochlea after exposure to difference levels of intense noise[J];Medical Journal of Chinese People's Liberation Army;2005-06
6 Yang Weiping, Yu Liming, Hu Yinyan et al. Institute of Otolaryngology, General Hospital of PLA, Beijing 100853, China;Time sequential analysis of the death of rat's cochlear outer hair cells at the early stage after exposure to impulse noise[J];Medical Journal of Chinese People's Liberation Army;2006-06
7 YANG Wei-ping, HU Bo-hua, Donald Henderson. Institute of Otolaryngology, General Hospital of PLA, Beijing 100853, China;The primary death pathway of outer hair cells of rat cochlea following styrene exposure[J];Medical Journal of Chinese People's Liberation Army;2009-07
8 YANG Wei-ping,YU Li-ming,HU Yin-yan,GUO Wei,CHEN Hong-wen(Institute of Otolaryngology,PLA General Hospital,Beijing 100853,China);Initial pattern of outer hair cell death in rat cochlea after exposure to impulse noise[J];Academic Journal of PLA Postgraduate Medical School;2007-01
9 FU Yong1 GONG Shusheng2 XUE Qiuhong1 WANG Guopeng1 CHEN Qingguo1(1Department of Otolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China;2Department of Otolaryngology, Tongren Hospital, Capital Medical University);Effect of the intense noise on hearing function and cochlea morphology in rat[J];Journal of Clinical Otorhinolaryngology Head and Neck Surgery;2008-11
10 FU Bu-qing1,ANG You-shun2 ,LU Si-wen1 , LIU Wen-hua3, ZHOU Yan-wu3 , PEI Yun3 , WU Kan2, XIE Zong-hua2,ZHU Ming-xue4,ZHAO Jie4 (1epartment of Blood Transfusion, Jiangsu Provincial Hospital of Traditional Chinese Medicine, Nanjing 210029, China; Department;Influence of Sulfur mustard on hemostatic and coagulative functions in rats[J];J0urnal of Nanjing Military Medical College;2001-04
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Yang Weiping, Hu Bohua, Donald Henderson. Institute of Otolaryngology, General Hospital of PLA, Beijing 100853, China;Death mode of outer hair cells in chinchilla cochlea after exposure to difference levels of intense noise[J];Medical Journal of Chinese People's Liberation Army;2005-06
2 Yang Weiping, Yu Liming, Hu Yinyan et al. Institute of Otolaryngology, General Hospital of PLA, Beijing 100853, China;Time sequential analysis of the death of rat's cochlear outer hair cells at the early stage after exposure to impulse noise[J];Medical Journal of Chinese People's Liberation Army;2006-06
3 Yang Weiping,Hu Bohua,Donald Henderson.Institute of Otolaryngology,General Hospital of PLA,Beijing 100853,China;Effects of mitochondrial energy-conversion efficiency on controlling the initiation and execution of the apoptotic process in outer hair cells of cochlea following noise exposure[J];Medical Journal of Chinese People's Liberation Army;2008-08
4 YANG Wei-ping,HU Bo-hua,Donald Henderson.Institute of Otolaryngology,General Hospital of PLA,Beijing 100853,China;Signal pathway associated with hair cell death in cochlea following exposure to intense impulse noise[J];Medical Journal of Chinese People's Liberation Army;2009-01
5 Chen Hongwen;Gu Rui;Zhai Suoqiang ; et a1.( General Hospital of PLA。 Beijing 10O853);EXPERIMENTAL STUDY ON ACOUSTIC ORGAN INJURIES BY INTENSE ACOUSTICIMPULSES[J];MEDICAL JOURNAL OF CHINESE PEOPLE'S LIBERATION ARMY;1995-02
6 YANG Wei-ping, HU Bo-hua, WANG Pei-ying, GUO Wei, JIANG Si-chang (Institute of Otolaryngology, PLA General Hospital, Beijing 100853, China);Technical improvement of chronic infusion of drugs into the guinea pig cochlea by an osmotic pump[J];Academic Journal of Pla Postgraduate Medical School;2001-01
7 YANG Wei-ping 1,HU Bo-hua 2 (1. Institute of otolaryngology, PLA General Hospital, Beijing 100853, China; 2. Center for Hearing and Deafness, Department of Communicative Disorders and Sciences, State University of New York at Buffalo, 137 Cary Hall, 3435 Main Street, Buffalo, NY 14214, USA);The method of detecting the apoptotic hair cells at their early stage in the cochlea[J];Academic Journal of Pla Postgraduate Medical School;2005-02
8 HU Bo hua,JIANG Si chang,GU Rui.Institute of Otorhinolaryngology, Chinese PLA General Hospital,Beijin 100853;Changes of Cochlear Microcirculation in Guinea Pigs Induced by Exposion[J];CHINESE JOURNAL OF TRAUMATOLOGY;1995-02
9 YANG Wei-ping1,HU Bo-hua21. Institute of Otolaryngology, PLA General Hospital, Beijing 100853, China2. Center for Hearing and Deafness, Department of Communicative Disorders and Sciences, State University of New York at Buffalo, 137 Cary Hall, 3435 Main Street, Buffalo, NY 14214, USA;Comparison of three methods for detecting the death modes of hair cells in the cochlea[J];;2004-04
10 YANG Wei-ping1, HU Bo-hua2 1 Institute of Otolaryngology, Chinese General Hospital of PLA, Beijing 100853, China 2 Center for Hearing and Deafness, State University of New York at Buffalo, 137 Cary Hall, 3435 Main Street, Buffalo, NY 14214, USA;The changes of succinate dehydrogenase activity in the apoptotic and necrotic outer hair cells following impulse noise exposure[J];Chinese Journal of Otology;2007-02
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