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《Chemistry and Industry of Forest Products》 2008-03
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Application of Molecular Imprinting Technology for Separation of Puerarin and Effect of Solvent on Recognition of Molecularly Imprinted Polymer

CHEN Li-na,DU Shu-hu,MA Kun-fang,CAI Zheng(School of Pharmacy,Nanjing Medical University,Nanjing 210029,China)  
A rational design approach was taken to the planning and synthesis of a molecularly imprinted polymer(MIP)capable of extracting puerarin from pueraria extract.The polymer was evaluated by static adsorption experiment.A molecular modeling approach was used to elucidate template-monomer interaction.Data from spectral analysis were used to prove the prediction made by molecular modeling.The MIPs have high selectivity to puerarin,the recovery rate of puerarin at 78.0 %,and purity at 86.5 % were attained.The successful preparation of molecular-imprinted polymers for solid-phase extraction for puerarin provides an innovative opportunity for the separation of traditional Chinese medicine.
【Fund】: 国家自然科学基金(30672532);; 江苏省高校自然科学研究计划项目(06KJD360128);; 南京医科大学科技发展基金重点项目(06NMUZ013)
【CateGory Index】: TQ461
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