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《Journal of Clinical Stomatology》 2005-01
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Cloning and sequencing of the coding region of mouse Dlx-5 gene

QU Tie-jun, WU Bu-ling, GAO jie, PANG Jian-liang.College of Stomatology, The Fourth Military Medical University, Xi'an 710032, China  
Objective:To clone the complete encoded sequence mouse Dlx-5 gene from mouse tooth germ, sequence and analyze its sequence.Method: Total RNA was isolated from embryo mouse mandible arch of 14 days and reverse-transcribed into cDNA.The desired cDNA was obtained by PCR. The segments was inserted into PMD-18 T vector and the positive clone was sequenced. Result: Dlx-5 cDNA was obtained from embryo mouse mandible arch. The sequence was consistent with that reported. One mutation base was found in this sequence.Conclusion: The Dlx-5 gene was sucessfully amplfied and sequenced. And this study provides the evidence of expression of Dlx-5 in embryo mouse mandible tissue.
【Fund】: 全军“十五”计划基金重点课题 (0 1Z0 89)
【CateGory Index】: Q785
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Chinese Journal Full-text Database 4 Hits
1 JIANG Bei-zhan,WANG Zuo-lin (Department of Oral and Maxillofacial Surgery,College of Stomatology,Tongji University, Shanghai 200072,China);Progress of the Related Signaling Molecules and Genes about the Initiation of Tooth Germ[J];Journal of Oral and Maxillofacial Surgery;2006-03
2 WU Li-an, WEN Ling-ying, YANG Fu-sheng, et al (College of Stomatology, The Fourth Military Medical University, Xi'an 710032, China);cDNA cloning and partial sequencing of USF1 in mouse tooth germ[J];Chinese Journal of Conservative Dentistry;2002-11
3 YU Qing (College of Stomatology, The Fourth Military Medical University, Xi′an 710032, China);Molecular mechanism of odontoblasts differentiation[J];Chinese Journal of Conservative Dentistry;2003-02
4 WU Li-an (College of Stomatology,The Fourth Military Medical University, Xi′an 710032, China);Role of HLH proteins in tooth development control[J];Chinese Journal of Conservative Dentistry;2003-05
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