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《Journal of Clinical Stomatology》 2005-01
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The study on oral epithelium primary culture with modified tissue culture in vitro

ZUO Zhi-bin, QI Xiang-min, ZHOU Wen-tao, YAN Shi-guo.College of Stomatology Shandong University,Jinan 250012,China  
Objective:To explore a new, prompt and simple human oral epithelium primary culture technique and to establish a reliable human oral epithelial cell culture system in vitro. Method:DispasedⅡ were used to separate epithelium from the underlying fibrous connective tissue. Modified culture system (serum free, 3T 3 cells free) was used to culture primary human oral keratinocytes with modified tissue culture technique in vitro. The difference of keratinocytes primary culture between traditional enzyme-digest culture and modified tissue culture was detected. Result:DispaseⅡ could successfully separate epithelium from the underlying fibrous connective tissue. Modified tissue culture technique could predigest experiment approaches and shorten operation time of primary culture, moreover, it could make primary keratinocytes subculture more quickly.Conclusions:Modified tissue culture technique was better than the enzyme-digest culture method in the primary epithelium culture.
【Fund】: 山东省科研基金资助项目 (Y2 0 0 1C2 6)
【CateGory Index】: R780.2
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