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《Journal of Clinical Stomatology》 2005-10
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Effects of static tension-force on the production of cyclooxygenase-2 by human periodontal ligament fibroblast

HUANG Sheng-gao,XIONG Pei-ying,ZHANG Jian-xing.Department of Stomatology,The Second XiangYa Hospital,Central South University,ChangSha 410011,China  
Objective:To study the effect of static tension-force on the expression of cyclooxygenase-2 by human periodontal ligament fibroblast(PDLFs).Method:Human PDLFs were treated by tension-force values of 0%,8%,13%,18% and 23% for 1 h,8 h,16 h,24 h respectively with tensional apparatus in vitro.After treatment each sample was collected and measure the expression of cyclooxygenase-2 by using immunohistoche staining.and determination.Result:The expression of cyclooxygenase-2 added with the increase of loading time and tension-force value in the group of 8%,13%,18%.The expression of cyclooxygenase-2 was depressed in group of 23% and had no relation with the loading time.Conclusion:The tension-force of physiological range(8%,13%,18%) stimulates the expression of cyclooxygenase-2 by human PDLFs,when the tension-force is over physiological range(23%),it will depressed the expression of cyclooxygenase-2.
【Fund】: 湖南省自然科学基金资助项目(03jjy4033)
【CateGory Index】: R783
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