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《Journal of Clinical Stomatology》 2005-10
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The effects of Salvia Miltiorrhizae on the apoptosis of vascular endothelial cells induced by arecoline

YIN Xiao-min,PENG Jie-ying,GAO Yi-jun,FANG Chang-yun,PANG Dan-lin,XIA Yu.Department of Stomatology,Xiangya Hospital,Central South University,Changsha 410078,China  
Objective:To explore the protective effects of Salvia Miltiorrhizae(SM) on the apoptosis of vascular endothelial cells(VEC) induced by arecoline.Method:Human Umbilical Vein Endothelial Cells(HUVECs) were cultured in vitro.Apoptosis of VEC were induced by arecoline,and SM was added in test specimen.Apoptotic morphological changes and percentage of apoptosis were assayed with Hoechst 33258 staining and were observed under fluorescent microscope.Result:There was relatively less number of apoptosis of in vitro cultured normal VEC than that after arecoline stimulation.After treatment with SM,VEC apoptosis decreased significantly(P0.05).Conclusion:SM might exert protective effects on VEC against apoptosis induced by arecoline.
【Fund】: 湖南省科技厅发展基金资助项目(02SSY3052)
【CateGory Index】: R285;
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