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Experimental study on the dynamic change of telomerase activity during golden hamster cheek porch carcinogenesis

GE Shu-yun,LOU Jia-ning,ZHOU Zeng-tong.Department of Oral Medicine,Shanghai Research Institute of Stomatology,Shanghai Key Laboratory of Stomatology,School of Stomatology The Ninth People's Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 200011,China.  
Objective:To study the dynamic regularity of telomerase activity during Syrian golden hamsters cheek pouch carcinogenesis induced by 7,12-Dimethylbenz alphaanthracene(DMBA).To provide the baseline for possible mechanism of Oral Leukoplakia(OLK)carcinogenesis.Method:Salley approach was adopted to produce the model of OLK carcinogensis.55 golden hamsters,ranging in age from 4 to 8 weeks old and weighing 80 to 120 grams,were divided into 2 groups:In group A(the control),5 hamsters were sacrificed from 4 to 8 weeks.In group B(the model),50 hamsters were painted bilaterally on the cheeks with 0.5% DMBA 3 times a week,establishing an experimental OLK group,and were sacri-ficed from 4 to 8 weeks.Double blind protocol was applied to judge the dynamic pathologic changes.TRAP-ELISA protocol was used to examine the telomerase activity.Result:The rate of telomerase positive in control and model group were 0% and 52.27%.there were significant difference between each pathologic stage of carcinomatous change of OLK(p0.05 or p0.01).Conclusion:Ttelomerase could act as a surrogate endpoint biomarker for oral chemoprevention during OLK carcinogenesis.
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