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Effect of concentrations of sucrose on Streptococcus mutans wapA expression

JIANG Ying1,ZHANG Tie-zhu1,YANG Jin-bo2,LIU Tian-jia2,LAN Qing1.Affiliated Hospital of Guangdong Medical College,Guangdong Zhanjiang 524001,China.2.West China College of Stomatology,Sichuan University.Sichuan Chengdu 610041,China.  
Objective:To detect the influence of concentrations of sucrose on expression of wapA,the adherence as-sociated gene of S.mutans.Method:Total RNA was extracted from S.mutans after being cultured in 0.5%,1.0%,5.0% su-crose and then reversely transcripted to cDNA.By utilizing TaqMan RT-RCR technique,we checked wapA expression.Re-sult:In different concentrations of sucrose,wapA expression didn't changed significantly;In 0.5% and5.0% sucrose,wapA expression of more-adherent S.mutans strains was significantly higher than that of less-adherent strains.Conclusion:The supply of sucrose plays little effect on wapA expression;There might be some correlation between different expression of wa-pA gene and S.mutans sucrose-dependent adherence.
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