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《Journal of Clinical Stomatology》 2014-01
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Biocompatibility of smaIl intestinal submucosa and human dental pulp stem cells in vitro

ZHANG Hong-mei;HOU Li-ya;XUN Wen-xing;WANG Jiang;LI Rong;Qu Xiao-li;ZENG Guang;YANG Hai-zhen;Department of Stomatology,Tangdu Hospital,The Fourth Military Medical University;Department of Neurosurgery,Xijing Hospital,The Fourth Military Medical University;  
Objective:To observe the biological effects of small intestinal submucosa(SIS) on human dental pulp stem cells(DPSCs) cultured in vitro and investigate the odontogenic induction of DPSCs induced by SIS. Method: DPSCs were successfully isolated and cultured. The proliferation of DPSCs induced by SIS was evaluated by MTS assay. The odontogenic differentiation of DPSCs induced by SIS was measured using Alizarin red staining, the alkaline phosphatase(ALP) activity and reverse transcription-polymerase chain reaction(RT-PCR) assay. Result:The proliferation of DPSCs in SIS groups was obviously higher than that in the control groups. The higher levels of ALP activity and larger mineralizing nods were also detected in the experiment groups. RT-PCR demonstrated mRNA expression of dentin sialophosphoprotein(DSPP) and dentin matrix protein1(DMP-1) in DPSCs induced by SIS. Conclusion:SIS possesses a good cellular compatibility with DPSCs. As an engineering scaffold, it can induce the odontogenic differentiation of DPSCs.
【CateGory Index】: R363
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