Experimental study on lithium chloridein of distracted bone regeneration in rats
Zhang Nian;Wang Xue-mei;Li Yun-feng;He Dong-ming;Ji Huan-zhong;Luo En;State Key Laboratory of Oral Diseases,West China School of Stomatology Sichuan University;
Objective: To observe the effect of systematic administrated lithium on distraction osteogenesis in a rat model. Methods: Forty adult male Sprague-Dawley rats undergoing osteotomy of the right tibia diaphysis. Rats were divided equally into experiment and control groups. Lithium or saline treatment were administrated through gastric gavage until the rats were killed. Distraction( rate 0. 6 mm / day) began on the 7 th postoperative day continued for 14 days. Following 8 weeks consolidation period,the distracted tibias in both groups were harvested and examined by X-ray plain radiography,histology,dualenergy X-ray absorptiometry,Micro-CT and biomechanical tests. Results: Compared with the control group,there were higher bone mineral density,more mature new bone tissue,and better regenerated bone mass continuity in the distraction gaps in experiment group. Conclusion: Lithium treatment could increase bony callus ossification volume,accelerate distracted tissue mineralization and promote bone regeneration during distraction osteogenesis in the rat. Systemic administration of lithium may provide a feasible approach for promoting osteogenesis during distraction osteogenesis.