The expression of X-chromosome-linked inhibitior of apoptosis protein associated factor 1 in oral squamous cell carcinoma and its effect on cell growth and apoptosis of CAL27 cells
SHENG Xia-han;XU Xin;Department of Oral and Maxillofacial Surgery,The Second Affiliated Hospital Zhejiang University School of Medicine;
Objective: To explore the expression of X-chromosome-linked inhibitior of apoptosis protein associated factor 1( XAF1) in oral squamous cell carcinoma( OSCC) and investigate the effect of XAF1 overexpression on the proliferation,clone formation and apoptosis of CAL27 cells. Methods: The expression of XAF1 protein in 64 cases of oral squamous cell carcinoma and 14 normal oral mucosa tissues was detected by immunohistochemistry,and the correlation between the expression of XAF1 and the clinic opathological features of the patients was analyzed. CAL27 cells were transfected with XAF1 expression plasmid. Real-time PCR was used to detect the expression of XAF1 m RNA when transfected with 24 h. Inverted microscope was used to observe cell morphological changes. CCK8 and clones forming were used to detect the effect of XAF1 on cell growth. The effect of apoptosis was detected by flow cytometry and Western Blot. Results: The expression of XAF1 protein in OSCC was lower than that in normal oral mucosa. The expression of XAF1 was related to the TNM staging of the patients. Overexpression of XAF1 inhibited the proliferation and colony formation of CAL27 cells,and increased the percentage of apoptotic cells and cleaved-PARP. Conclusion: The expression of XAF1 in OSCC is decreased,which may be related to the progression of OSCC. Overexpression of XAF1 inhibits OSCC cell growth and promotes cell apoptosis.
【CateGory Index】： R739.8