The changes of type Ⅰ collagen expression in fibroblast-like cells from temporomandibular joint disc perforation
MA Zhuang;ZHANG Sheng;ZHU Yu-wen;HUNAG Hong-wei;Department of stomatology,Zhuzhou central hospital;
Objective: To study the changes of type Ⅰ collagen expression in fibroblast-like cells from temporomandibular joint disc perforation and investigate the effect of type Ⅰ collagen in the occurrence of temporomandibular joint disc perforation. Methods: The fibroblastic-like cells in experimental group from the surgical removed temporomandibular joint disc perforation tissue were cultured,and the cells from temporomandibular joint condyle hyperplasia were also cultured as the control group. The cultured cells were identified as fibroblastic-like cells by toluidine blue and immunofluorescence staining.The expression of intracellular type Ⅰ collagen was detected with Western Blotting and the content of extracellular type Ⅰcollagen was examined by enzyme linked immunosorbent assay. Results: Fibroblastic-like cells in two groups were positive by toluidine blue,and the collagen-Ⅰ and collagen-Ⅱ were positive by immunofluorescence staining. The content of extracellular collagen-Ⅰ was significantly higher than that in the control group( P 0. 05). The expression of typeⅠcollagen in cells from joints with disc perforation was lower than that from normal joints( P 0. 05). Conclusion: The expression level of collagen-Ⅰdecreases significantly in fibroblastic-like cells from temporomandibular joint disc perforation tissue,which may lead to the development and progression of temporomandibular joint disc perforation.
【CateGory Index】： R782.6