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The model of periodontitis was established by the combined use of alveolar nerve dissection,molar ligation and gingival crevicular fluid injection

LUO Yan-hua;ZHAGN Liang;LI Qing-yu;LU Mao-wei;LI Na;LI Yun;LONG Lu-yu;CHU Yan-e;Changsha medical college;  
Objective: To explore the method of establishing the mouse model for the experimental periodontitis,and to provide reference for the basic research. Methods: Fifty-four healthy mice were randomly divided into three groups that each group of 18 mice,blank group( the first molar and second molar clearance in mice injected saline),conditions,the control group( ligation silk oppression gums,at the same time from the inferior alveolar nerve broken grinding) and the experimental group( ligation silk oppression gums,from the inferior alveolar nerve broken grinding at the same time,cooperated with periodontitis patients with gingival sulcus liquid injection). The gingival index and tooth loosening composition,the serum Ig G,IL-4 and IL-6 contents and the local clinical features,morphology and pathological examination results of the gingiva of the three groups were recorded after six weeks feeding.Results: The difference in gingival index composition between the three groups was significant( P 0. 05). The three groups showed significant difference in the composition of tooth looseness( P 0. 05),which was derived from the difference between the experimental group and the blank group.After the study,Ig G,IL-4,IL-6 contents in serum of the three groups were significantly different( P0. 05),and the differences between the two groups were significant( P0. 05). In the experimental group,the mice showed obvious gingival redness and ulcer,and periodontal probe was prone to bleeding.The average crest of the mouse was about 1. 2 mm below the bone boundary.Pathological results indicated that a large number of osteoclasts damaged the structure of alveolar bone and alveolar crest,and there was plasma cell infiltration in the colleague tissue.Conclusion: The gingival space of the first molar and the second molar was injected into the gingival crevicular fluid of the patients with periodontitis,which was helpful to establish a typical model of periodontitis mice.
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