Effect of leukemia inhibitory factor on osteogenesis of human periodontal ligament cells
Liang You-de;FURun-ying;LIU Gen;YOU Xin;SONG Da-wei;Department of Dentistry,Seventh People's Hospital of Shenzhen City;
Objective: To probe the effect of leukemia inhibitory factor( LIF) on osteogenic differentiation of human periodontal ligament cells( HPDLCs).Methods: HPDLCs were harvested from orthodontic human and immunohistochemistry staining was employed for morphological observation.Three groups were set in the study including spontaneous differentiation group,bone induction group and LIF supplied bone induction group. After 7 days,alkalinephosphatase( ALP) staining and ALP activity were detected.The expression of ALP,BSP and OCN mRNA was measured by qRT-PCR.After 21 days,mineralized nodules were detected by alizarin red staining. Results: ALP staining appeared a dark blue in the osteogenic induced group,while much weaker in the group treated with LIF.The activity of ALP was significantly higher in the osteogenic induced group than that in the group stimulated by LIF( P 0. 01).Alizarin red staining showed lots of mineralized nodules in the osteogenic induced group,while the much less in the group treated with LIF( P0. 05).The mRNA expression of ALP,BSP and OCN was significantly higher in the osteogenic induced group compared to that in the group treated with LIF( P0. 01).Conclusion: LIF inhibited osteoblastic differentiation of HPDLCs in this study.