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《The Journal of Clinical Anesthesiology》 2013-01
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The effects of Protectin D1 on the adhesion of neutrophils and human umbilical vein endothelial cells

WANG Yan, YAO Shang-long, SHU Hua-qing, GUO Li-na, WU Xiao-ying, WANG Ya-xin.Department of Anesthesiology, Union Hospital Affiliated to Huazhong University of Science and Technology, Wuhan 430022, China  
Objective To investigate the effects and mechanism of PD1 on the adhesion of neutrophils (PMNs) and human umbilical vein endothelial cells (HUVECs) induced by lipopolysaccharide (LPS). Methods Human umbilical vein endothelial cells(HUVECs)cultured in vitro was randomly divided into five groups for the experiment of PMNs-HUVECs adhesion.There were group P2 , group P20 , group P200 ,group LPS, group control(group C). LPS (1 μg/ml) was used to stimulate HUVECs for 6 h except the group C. BCECF-AM labeled PMNs were added into the HUVECs monolayer for another 30 min. PMNs adhesion was detected by fluorescence microscope. Flow cytometry was applied to assess lymphocyte function-associated antigen (LFA-1) expression.Western blotting was used to detect the intercellular adhesion molecuh-1(ICAM-1) expression. Results Compared with group C,the fluorescence intensity in group P2,P20 ,P200 and LPS were significantly increased ( P 0.05).Compared with group LPS, the PMNs-HUVECs adhesion of P2 ,P20, P200 were significantly decreased( P 0.05),and the PD1 inhibited the adhesion of PMNs to HUVECs in a concentration-dependent manner. Compared with group C,the expression of LFA-1 in group P2,P20 ,P200 and LPS were obviously increased ( P 0.05),while the group P20 and P200 were lower than group LPS( P 0.05).PD1 suppressed LFA-1 expression in PMNs( P 0.05) in a concentration-dependent manner without any significant effect on ICAM-1 expression in HUVECs. Conclusion PD1 can inhibit the adhesion of PMNs and HUVECs in a concentration-dependent manner. It may be related to the decrease of LFA-1 expression in PMNs.
【Fund】: 湖北省卫生厅项目(JX4C25)
【CateGory Index】: R965
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