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《The Journal of Clinical Anesthesiology》 2013-01
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Protective effects of sevoflurane postconditioning on oxygen-glucose deprivation injury in rat hippocampal slices

ZHANG Yong-hua, CHEN Qiu-ping, HUANG Xue-lian, CAO Su.Department of Anesthesiology, Affiliated Hospital of Nantong University, Nantong 226001, China  
Objective To investigate the protective effects of sevoflurane postconditioning on oxygen-glucose deprivation injury in rat hippocampal slices. Methods Forty hippocampal slices were randomly divided into 4 groups ( n =10 each): group OGD; group 2% Sev; group 4% Sev; group 6% Sev. The technique of electrophysiology was used, and the amplitude of orthodromic population spike (OPS) in the stratum pyramidale of the CA1 region was measured. And TTC staining was used to calculate the percentage of tissue injury. Results OPS recovery amplitude was (3.14±2.56)%, OPS recovery rate was 10%, and the degree of tissue injury was 0.63±0.05 in group OGD. OPS recovery amplitude was (46.24±29.34)%,OPS recovery rate was 40%, and the degree of tissue injury was 0.51±0.06 in group 2% Sev. OPS recovery amplitude was (62.40±34.93)%,OPS recovery rate was 60%, and the degree of tissue injury was 0.41±0.07 in group 4% Sev. OPS recovery amplitude was (75.92±45.31)%,OPS recovery rate was 70%, and the degree of tissue injury was 0.37±0.06 in group 6% Sev. The degree and rate of recovery of OPS were increased but the tissue injury was deceased in group 2% Sev, group 4% Sev and group 6% Sev as compared with OGD group. Conclusion Sevoflurane postconditioning can protect neurons from oxygen-glucose deprivation injury in rat hippocampal slices.
【CateGory Index】: R96
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