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Genetic Analysis of 102 Cases with Chronic Myelogenous Leukemin

DING Nanhua (department of function center,Jiangxi Medical college,Nanchang 330006,China)  
Objective:To investigate the value of detection of Ph chromosome and bcr/abl fusion gene in the diagnosis of CML.Methods:Bone marrow direct method and/or short-term culture were used to prepare the chromosomes.bcr/abl fusion gene was analyzed by RTnestPCR method.Results:Ph chromosome and bcr/abl fusion gene were both positive in 91 patients, Ph -/bcr/abl + in 6 patients, and Ph -/bcr/abl -in 5 patients,respectirely.Conclusion:Both Ph chromosome and bcr/abl fusion gene are important figures in diagnosis of CML and combining them is more evaluable.
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