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《Practical Clinical Medicine》 2003-01
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The Clinical Research on Detection of Hepatitis B Virus by Fluorescence Quatitative PCR

Zhu Shui shan 1, Yu Rong 2, Liu Jing mei 1, Ye Ru mei 1 (1.Department of Gastroenterology,2.Department of Endocrinology, Second Affiliated Hospital of Jiangxi Medical College,Nanchang 330006,China)  
Objective: To set up a Fluorescence quatitative PCR assay(FQ PCR)to explore the clinical value of detecting the load of HBV DNA. Methods: FQ PCR technique was used to measure the loads of HBV DNA of the cases of hepatitis B and the cases of HBV antigen or antibody negative, and 30 cases of chronic hepatitis B treated with Lamivudine.Results:(1)The positive rate of HBV DNA was closely correlated to that of HBeAg,and there was 3% positive rate of the DNA in the cases of HBV antigen or antibody negative.(2)The loads of HBV DNA in patients with chronic hepatitis B or cirrhosis were significant higher than that in ones of HBV healthy carriers and in patients with acute hepatitis B( P 0.01).(3)The rate of HBV DNA turned to negative was 73.3%(22/30),but only 46.2% of HBeAg positive samples turned to negative( P 0.05)after 12 months treating with Lamivudine in 30 cases of chronic hepatitis B.Conclusion:FQ PCR was an accurate quatitative technique in detecting HBV DNA and it was significant better than ELISA technique in diagnosis of hepatitis B and selection of anti virus drugs.
【CateGory Index】: R446.5
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【Co-references】
Chinese Journal Full-text Database 10 Hits
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