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《Chinese Journal of Clinical Medicine》 2006-01
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Experence of A New Abdominal Cardiac Transplantation Model in Rats

LIU Fabing LIU Zhihong TAN Jianming, et al.Department of Renal Transplantation,Shanghai First People's Hospital; Shanghai Clinical Medical Center of Organ Transplantion,Shanghai 200080  
Objective:To establish an improved heterotopic cardiac transplantation model in rats. Methods:Sprague Dawley rats have been used as recipients and Wistar rats as donors. Donor aorta was anastomosed to the recipient's suprarenal abdominal aorta;donor pulmonary artery was anastomosed to the left renal vein of the recipient using continuous suture technique The operation was considered be successful if the recipient rat and graft survive over 3 days. They were compared with models performed using modified Ono procedure Results: Forty rats of heterotopic cardiac transplantation were performed using new procedure and twenty were performed using modified Ono procedure, with successful rate 92. 5% and 75% respectively. Mean time of ischemia and operation was both significantly shorter in rats using new procedure than that of rats using modified Ono procedure The rate of complications in rats using new procedure was significantly lower. Conclusion:The method is easy,quick and reliable, which has a low incidence of morbidity and mortality. The model perhaps is more suitable for heterotopic heart transplantatioa
【Fund】: 上海市卫生局青年科研基金资助项目(044Y20)
【CateGory Index】: R-332;R654.2
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