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《Chinese Journal of Clinical Medicine》 2006-01
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Study on Intracellular Calcium Overload and Intestinal Epithelial Cell Apoptosis

CHEN Qian QIN Huanlong Department of Surgery, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiaotong University, Shanghai 200233  
Objective:To study the influence on the IEC apoptosis with ischemia and anoxia injury, and the relationship of intracellular calcium amount and the effects of calcium channel antagonist on apoptosis. Methods: Construction of model of IEC apoptosis induced by ischemia and anoxia,IECs were divided into eight groups, namely, A1(control group), B1(anoxia group).C1(ischemia group),D1(ischemia and anoxia group);A2 (A1+ 2mg/l verapanil group), B2(B1 + 2mg/ 1 verapanil), C2(C1 + 2mg/l verapanil), D2 (D1+2mg/l verapanil). The apoptosis rate was determined by FCM, intracellular calcium amount was measured by LSCM. Results:The Intracellular calcium and the apoptosis rate (AR) increased in B1 ,C1 ,D1 group as compared with group A1,P0.01, AR in group D was higher than that in group B1,C1, P0.05) The AR were inhibited and decreased after using the calcium channel antagonist (verapanil), and the AR in group D2 improved markedly as compared with other groups. Conclusion:Intracellular culcium overload is relative to the apoptosis of IEC under ischemia and anoxia) calcium channel antagonist (verapanil) could decrease intracellular calcium and apoptosis rate of IEC.
【Fund】: 国家自然科学基金(批准号30271286) 上海市科委青年科技启明星跟踪计划(批准号02QMB1406)资助项目
【CateGory Index】: R363
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