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《Chinese Journal of Clinical Medicine》 2006-02
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Pulmonary Keratinocyte Growth Factor Production during LPS-Initiated Lung Inflammation in Endotoxin Tolerant Rats

PAN Jue ZHANG Jing QU Jieming,et al.Department of Pulmonology,Zhongshan Hospital,Fudan University,Shanghai 200032  
Objective:To study the protein production of keratinocyte growth factor (KGF) in endotoxin tolerant rats. Methods: Forty Sprague-Dawley male rats were randomized into endotoxin tolerance (ET) group and normal control (NO group. Endotoxin tolerance was induced by four daily intraperitoneal injections of 0.6 mg/kg/day Escherichia coli LPS (serotype 055; B5). NC group received intraperitoneal injections of the same volume saline. On the fifth day, rats were injected with high dose of LPS (6 mg/kg) to induce endotoxemia and lung inflammation. Lung tissue was collected before and 2, 6, 24 hours after the high dose injection of LPS (five rats for each time point). The protein production of KGF was measured by ELISA Software SPSS 11.5 was utilized for statistical analysis. Results: Before high dose LPS challenge, KGF production in lung tissue was higher in ET group (35.6±5.0) pg/ml than in NC group (24.2±5.3) pg/ml, P=0.009. After the challenge of high dose LPS, the level of KGF rapidly decreased in NC group, reaching its nadir with (5.0±1.4) pg/ml at 6 hours after injection (P=0.001). As contrasted, the KGF level in ET rats decreased more slowly and the value was significantly higher than that of NC group at 2 h and 6 h. Conclusion: The induction of endotoxin tolerance is associated with a higher level of KGF in lung tissue, which might be protective against severe lung injury initiated by endotoxemia.
【Fund】: 上海市科委基础研究重点项目(01JC14016) 国家自然科学基金资助课题(30371341)
【CateGory Index】: R563
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【References】
Chinese Journal Full-text Database 1 Hits
1 ZHU Minfang~1 QU Jieming~(1.2) ZHANG Huijun~1 ZHOU Shuancheng~3 ZHANG Jing~1 DONG Shengfu~3.1.Department of Pulmonology,Zhongshan Hospital,Fudan University,Shanghai 200032;2.Shanghai Huadong Hospital,Shanghai 200040;The Study on the Role of Growth Factor Independence 1 in the Lungs of Endotoxin Tolerance Mice[J];Chinese Journal of Clinical Medicine;2007-01
【Co-citations】
Chinese Journal Full-text Database 2 Hits
1 WANG Wei 1, WEI Jing Guo 1, WEI Long Xiao 1, CHUI Guang Bin 1, LU Zou 1, ZHANG Wei 2, LI Qi 3 1Department of Radiology, 2Department of Pathology, Tangdu Hospital, Xi'an 710038, China; 3Department of Immunology, Faculty of;Active expression of EGF and TGF-β_1 and pathological grading of pulmonary alveolitis in early bleomycin induced pulmonary injuries[J];Journal of The Fourth Military Medical University;2001-14
2 CAI Ying1,WU Qing-hua2(1. Department of Respiratory Medicine,The First Affiliated Hospital of Guangxi Medical University,Guangxi530021;2. Department of Respiratory Medicine,Beijing Hospital of Integrated Traditional and Western Medicine,Beijing 100039);Progresses of non-ventilation treatment for acute lung injury[J];World Clinical Drugs;2009-06
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