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《Chinese Journal of Clinical Medicine》 2006-02
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The Expression of Endothdin and Endothelin Receptors in Systemic and Pulmonary Circulation in Rabbit

YE Ming JIA Bing CHEN Zhanggen et al.Cardiovascular Center,Institute of Children,Department of Pathlolgy,Children's Hospital,Fudan University,Shanghai 200032  
Objective: To investigate the expression of endothelin and endothelin receptors in systemic and pulmonary circulation. Methods: 12 Newsealand rabbits, body weight is around 3 - 4 kg. The pulmonary artery and renal artery 1.5cm, pulmonary tissue and renal tissue 1.5cm×1. 5cm were excised. We determined the expression of endothelin by reverse transcriptase - polymerase chain reaction (RT-PCR), ELISA and immunohistochemical analysis. Results: The expression of endothelin A receptor in pulmonary artery is higher than in the renal artery (P0.01). There is no difference betweem the other groups. Conclusion:The expression of endothelin A receptor in pulmonary artery is higher than in the renal artery. There is no significant difference between the other tissues of endothelin receptor. There are strong expression of endothelin receptor in tracheal. It is show that there are closely related with endothelin and pulmonary function.
【CateGory Index】: R329
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【References】
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【Co-references】
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