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《Chinese Journal of Clinical Medicine》 2006-02
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Hexamethylene Bisacetamide's Effect of inducing HL60 Cells Differentiation and Apoptosis

CHEN Zi FAN Huahua MENG yan,et al.Department of Hematology,Huashan Hospital,Fudan University,Shanghai 200040  
Objetive:To investigate the effect of hexamethylene bisacetamide (HMBA) on differentiation and apoptosis of HL60 cells in vitro. Methods:Differentiation of HL60 cells was detected by the change of antigen expression of CD11b,CD14 and CD68 on cell surface. The staining of Annexin V/PI was used to detected the apoptosis of HL60 cells. The changed cell cycles distribution of HL60 cells induced by HMBA was analysed by flow cytometry. Results : HMBA could induce the increased expression of CD11b of HL60 cells, which showed HL60 cells differentiation into granular line. At the same time, HMBA could induce HL60 cells trivial apoptosis. HMBA could commit HL60 cells to G0/G1 phase arrest. Conclusion: Inducing differentiation is the primary effect of HMBA on HL60 cells, and inducing apoptosis is not. HMBA can arrest HL60 cells at G0/G1 phase, which may be essential to the effect of HMBA on HL60 cells.
【CateGory Index】: R733.7
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【References】
Chinese Journal Full-text Database 2 Hits
1 MENG Yan1, DIANG Tian-ling2, ZHU Ping2, XIE Yi2 1.Shanghai Blood Center, Shanghai 200051, P.R.China 2.Department of Hematolgy, Huashan Hospital, Fudan University, Shanghai 200040, P.R.China;Research of hexamethylene bisacetamide’s effect on K562 cells in vitro[J];Chinese Journal of Cancer Prevention and Treatment;2007-14
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【Co-references】
Chinese Journal Full-text Database 7 Hits
1 WANG Zhe 1, QIAO Yan 2, HUANG Gao Sheng 1, WANG Ai Qin 2, ZHANG Yong Qing 1, FENG Ji Liang 1, YANG Guo Rong 1, GUO Ying 1, LIANG Rong 1 1Department of Pathology, Faculty of Predinical Medicine, Fourth Military Medical University, Xian 710033, China, 2Lanzhou Chemistry Physics Institute, Chinese Academy of Sciences, Lanzhou 730000, China;Induction of macrophagic differentiation of leukemia cell line K562 by N-acetyl-D-glucosamine[J];Journal of The Fourth Military Medical University;2003-01
2 CHEN Zi YANG Yongchen GAO Li FAN Huahua XIEYanhui XIEYi (Department of Hematology, Huashan Hospital, Fudan University, Shanghai 200040, China; Shanghai Blood Center);The research of hexamethylene bisacetamide's effect on HL60 cells[J];Journal of Clinical Hematology;2006-04
3 YANG Jian,MI Man tian,YANG Zhi xiang,WEI Na,WANG Fang Department of Nutrition Higiene,Third Military Medical University,Chongqing 400038,P.R.China;Effects of anti-sense cyclin D1 inducing in retinoic acid dependent manner on the proliferation and differentiation of HL-60 cells and its mechanism[J];Journal of Qilu Oncology;2004-02
4 OUYANG Gao Liang LI Qi Fu PENG Xuan Xian HONG Shui Gen (Laboratory of Cell Biology, The Key laboratory of Ministry of Education for Cell Biology and Tumor Engineering, School of Life Sciences, Xiamen University, Fujian Xiamen 361005);EFFECTS OF HMBA ON THE EXPRESSION OF CELL-CYCLE-ASSOCIATED GENES IN HUMAN HEPATOCARCINOMA SMMC-7721 CELLS[J];Acta Biologiae Experimentalis Sinica;2002-03
5 WU Li De, CHEN Yuan Zhong, LI Nai Nong, WU Yong (Fujian Institute of Hematology, Union Hospital of Fujian Medical University, Fuzhou 350001, China);Study on Telomerase Activity and Expression of hTERT, c-myc and bcl-2 during Terminal Differentiation of HL-60 Cells Induced by Retinoic Acid[J];Journal of Experimental Hematology;2002-05
6 WANG Qin-Hong, XIE Yi, FAN Hua-Hua 1, GAO Li 1, LIU Yan 1 Department of Hematology, Huashan Hospital, Fudan University, Shanghai 200040, China; 1Blood Engineering Lab, Shanghai Blood Center, Shanghai 20 0051, China;Effects of Hexamethylene Bisacetamide on Cell Cycle and Expression of Its Regula tory Proteins in HL-60 Cells[J];Journal of Experimental Hematology;2003-05
7 WANG Qin-hong ,XIE Yi,Fan Hua-hua,GAO Li,LIU Yan,XIE Yan-hui. *Haematology Department,Fudan University Affiliated Huashan Hospital,Shanghai 200040,China;Effect of HMBA on differentiation and apoptosis of HL-60 and U937 cells and its mechanism[J];Chinese Journal of Hematology;2004-03
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