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《Chinese Journal of Clinical Medicine》 2017-03
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Screen of time window of stable expression of enhanced green fluorescent for transplanted hepatocellular carcinoma in Buffalo rats

ZHANG Wei;QIAN Sheng;ZHU Liang;ZHOU Bo;YANG Guo-wei;LIU Rong;QU Xu-dong;WANG Jian-hua;YAN Zhi-ping;Department of Intervention Radiology,Zhongshan Hospital,Fudan University,Shanghai Insitute of Medical Imaging;  
Objective:To discuss the time window of stable expression of enhanced green fluorescent for transplanted hepatocellular carcinoma in Buffalo rats.Methods:McA-RH7777/EGFP cells were subcutaneously implanted into the Buffalo rats' right thighs.Pieces of tumor tissue were implanted into the left lobes of the livers in 35 rats.The weights and serum albumin levels of rats were measured on day 7,12,18,21,26 after tumor implanted.The subsequent growth,metastasis of the implanted tumors were observed and evaluated with whole-body fluorescence optical imaging system,fluorescent microscopy.Results:The tumors were detected with MRI in all animals on day 7after tumor implanted.There was no significant growth of weight within 7days,but increased significantly after and reached the peak value at day 21,then decreased.The mean tumor volumes was(13.36±2.90)mm3 on day 7,(162.5±69.71)mm3 on day 12,increased to(1 683.36±375.02)mm3 on day 26 after tumor implanted.With the fluorescent microscopy,micrometastasis tumors can be detected in lungs on day 21 after tumor implanted.Multiple small lung metastases were detected on day 26.The albumin levels on day 26 after tumor implanted were significantly lower than before(P0.05).Conclusions:The period from 12 to 18days in the models may be classified as the micrometastasis stage,and it becomes advanced liver cancer after 21 days.
【Fund】: 国家自然科学基金(81171432);; 复旦大学附属中山医院人才培养计划资助项目(2015ZSYYYXQN06 2015ZSYXGG18)~~
【CateGory Index】: R735.7
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【Citations】
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1 ZHANG Wei;QIAN Sheng;QU Xudong;ZHU Liang;ZHOU Bo;YANG Guowei;WANG Jianhua;LIU Rong;YAN Zhiping;Deptartment of Interventional Radiology,Zhongshan Hospital,Fudan University;;Expression of E-cadherin in Rat Model of Hepatic Carcinoma during Transarterial Embolization and Its Relationship with Pulmonary Metastasis[J];中国临床医学;2015-01
【Co-citations】
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1 ZHANG Wei;QIAN Sheng;ZHU Liang;ZHOU Bo;YANG Guo-wei;LIU Rong;QU Xu-dong;WANG Jian-hua;YAN Zhi-ping;Department of Intervention Radiology,Zhongshan Hospital,Fudan University,Shanghai Insitute of Medical Imaging;;Screen of time window of stable expression of enhanced green fluorescent for transplanted hepatocellular carcinoma in Buffalo rats[J];中国临床医学;2017-03
【Co-references】
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1 ZHANG Wei;QIAN Sheng;ZHU Liang;ZHOU Bo;YANG Guo-wei;LIU Rong;QU Xu-dong;WANG Jian-hua;YAN Zhi-ping;Department of Intervention Radiology,Zhongshan Hospital,Fudan University,Shanghai Insitute of Medical Imaging;;Screen of time window of stable expression of enhanced green fluorescent for transplanted hepatocellular carcinoma in Buffalo rats[J];中国临床医学;2017-03
2 DUAN Xue-qing;ZHENG Li-ru;LIU Wen-bo;HOU Xu-gang;GUO Yu-yu;XIE Fang-rui;WANG Wen-qian;AN Hai-long;State Key Laboratory of Crop Biology, College of Life Sciences,Shandong Agricultural University;;Production of Transgenic Maize Plants via the GFP Green Fluorescence-assisted Selection[J];玉米科学;2017-02
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