Determination of paracetamol in human plasma by solid phase extracted HPLC method
Chen Miaoying, Zhong Mingkang, Shi Xiaojin, Zhang Lili, Wang Hongtu, Zhang Jinghua, (Department of Pharmacy, Huashan Hospital, Fudan University, Shanghai 200040)
AIM: To establish a new high performance liquid chromatographic (HPLC) method for determination of paracetamol in human plasma, and apply to study of the relative bioavailability of paracetamol. METHODS: Aminophylline was used as internal standard (IS). Plasma (0 2 ml) was extracted by a C 2 solid phase extraction (SPE) cartridge. Mobile phase was methanol: acetonitrile: water (25∶15∶360). RESULTS: The minimum detective concentration of paracetamol was 0 05 μg/ml, and concentrations range of standard curve was from 0 20 μg/ml to 15 0 μg/ml. Average within run precision and between run precision were near 6%. Recoveries of the method were almost 100%, with relative standard deviation (RSD)5%. CONCLUSION: The method can be applied to study the relative bioavailability of paracetamol.
【CateGory Index】： R96