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《Chinese Journal of Clinical Pharmacy》 2006-01
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Effects and mechanisms of curcumin on apoptosis of lung adenocarcinoma A549 cells

Tian Dezeng,Zhu Hui,Liang Yongjie(Department of Respiratory,Dongfang Hospital,Tongji University,Shanghai 200120,China)  
AIM To explore the effect of curcumin on inhibiting the apoptosis of the lung adenocarcinoma A549 cells and related mechanism.METHODS The inhibiting ratio of curcumin in different concentrations was determined by MTT assay at different times;The apoptosis rates of A549 cells cultured with 40 μmol·L~(-1) curcumin were calculated by Flowcytometry.The Caspase-9 expression of each group was determined by Flowcytometry.The Caspase-8 and Caspase-3 expression of each group were determined by colorimetry set.The Caspase-3 expressions of curcumin groups added Caspase-9 inhibitor were determined in the same way.Statistical method: χ~2 test or t test with SPSS10.0 software.RESULTS The inhibition of curcumin on lung adenocarcinoma A549 cells was shown in the dose-dependent and timedependent manner(P0.01).The apoptosis rate of A549 cells induced by curcumin(30.25%)was remarkably higher than the control(1.32%)(P0.001).The expressions of Caspase-3,-8,-9 induced by curcumin were obvious compared with the control(P(0.001)).After using Caspase-9 inhibitor,the expression of Caspase-3 decreased remarkably(P(0.001)),but it was more than control group(P0.001).CONCLUSION Curcumin can activate the expression of Caspase-8,Caspase-9 and Caspase-3 and induce the apoptosis of lung adenocarcinoma A549 cells through mitochondrion-depended and mitochondrion-undepended pathway.
【CateGory Index】: R734.2
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