Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Clinical Pharmacy》 2006-01
Add to Favorite Get Latest Update

Determination of methylprednisolone in plasma of guinea pig

Li Fang,Hu Yanfeng,Zhang Jian(Department of Pharmacy,Xinhua Hospital,Medical College,Shanghai Jiaotong University,Shanghai 200092,China)  
AIM To establish a method for determination of methylprednisolone in plasma of guinea pig.METHODS Methylprednisolone was determined by HPLC using ZORBAX Eclipse XDB-C_(18) column(250mm×4.6mm,5μm),7.56 mmol·L~(-1) ammonium sulfate-acetonitrile(68∶32,V/V) as mobile phase with a flow rate of 1.0 mL·min~(-1).The temperature of column was 20℃,the wavelength of detection was 243 nm.RESULTS The calibration curve was linear within the concentration range of 0.10-5.0 mg·L~(-1)(r=0.999 8),the limit of detection was 0.04 mg·L~(-1),the relative standard deviation values of within-day and between-day analysis were 1.91% and 2.57%,respectively.Two hours after administration,the concentration of methylprednisolone was 1.67 mg·L~(-1) in plasma.CONCLUSION The method is sensitive,accurate,reproducible and can be employed for the determination of the concentration of methylprednisolone in plasma.
【CateGory Index】: R96
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 1 Hits
1 HU Jian, LI Chong wei, ZHANG Xuan, PI Shi hong, ZHAO Jin sheng, CHEN Xiao ying, LIU Yan Group of Rheumatology and Immunology, Department of Internal Medicine, Tianjin Children′s Hospital, Tianjin 300074, China;Methylprednisolone and cyclophosphamide pulse therapy of severe systemic lupus erythematosus in children[J];Chinese Journal of Pediatrics;2003-06
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 ZHOU Wen-yan 1, ZHAO Shao-ling 2 (1.Hefei Kangcheng Technology Ltd,Hefei 230041,China;2.The People's Hospital of Lai'an County,Lai'an 239200,China);HPLC determination related compounds of erthylenediamine diaceturate injection[J];Anhui Medical and Pharmaceutical Journal;2005-01
2 ZHANG Yuyun, ZHENG Wenping ,ZHENG Yihui, et al Department of pharmacy,the Second Affiliated Hospital,Medical College of Shantou University,Shantou 515041,China;Study on Stability of Compatibility of Cefuroxime Sodium Injection with 6 Drugs[J];Journal of Medical Forum;2004-07
3 JIANG Yun-ping,ZHANG Li-na,HAI Hui,Chengdu Military General Hospital,Chengdu 610083;Stability of Doxofylline Injection Combined with Metronidazole Injection[J];Journal of Military Surgeon in Southwest China;2005-03
4 Hao ZhiYun; et al;EXPERMENT STUDAY OF STABILITY ON THE VITAMIN C[J];JOURNAL OF MUDANJIANG MEDICAL COLLEGE;1997-01
5 YANG Jing,WU Xiao-na (Department of Clinical Laboratory,Sichuan Provincial People's Hospital,Chengdu 610041,China);Determination of Vitamin C in Serum by HPLC[J];Journal of Modern Laboratory Medicine;2007-05
6 ZHANG Shu qiu,LENG Guang,ZHANG Li feng (School of Pharmacy, Shanxi Medical University,Taiyuan 030001, China);In vitro study on interaction between levofloxacin hydrochloride for injection and its co-administrated drugs[J];Journal of Shanxi Medical University;2002-01
7 ;沐舒坦与氨茶碱配伍使用应注意的问题[J];Journal of Practical Nursing;2004-10
8 Liu Lifang,Lu Zhihan(Guangzhou Municipal Institute for Drug Control,Guangzhou 510160);Determination of Medrol and Medrol Hemisuccinate in Solu-medrol SterilePowder by RP-HPLC[J];JOURNAL OF INSTRUMENTAL ANALYSIS;1998-04
9 WUWei_wen,YUWei_biao (The People's Hospital of Shunde District of Foshan,Guangdong528300,China);Investigation of the stability of kaki dehydrographollidi sauciness matched with18kinds of medicine[J];Modern Medicine Health;2005-10
10 YANG Cong lin,LOU Hong bing ,ZHANG Kai zheng (The People's HospitaL of Yiwu,Yiwu ZHEJIANG 322000,China);Compatible stability of vitamin C and tinidazole by dual wavelength spectrophotometry[J];Chinese Journal of New Drugs and Clinical Remedies;2003-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved