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《Chinese Journal of Clinical Pharmacy》 2006-01
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Determination of methylprednisolone in plasma of guinea pig

Li Fang,Hu Yanfeng,Zhang Jian(Department of Pharmacy,Xinhua Hospital,Medical College,Shanghai Jiaotong University,Shanghai 200092,China)  
AIM To establish a method for determination of methylprednisolone in plasma of guinea pig.METHODS Methylprednisolone was determined by HPLC using ZORBAX Eclipse XDB-C_(18) column(250mm×4.6mm,5μm),7.56 mmol·L~(-1) ammonium sulfate-acetonitrile(68∶32,V/V) as mobile phase with a flow rate of 1.0 mL·min~(-1).The temperature of column was 20℃,the wavelength of detection was 243 nm.RESULTS The calibration curve was linear within the concentration range of 0.10-5.0 mg·L~(-1)(r=0.999 8),the limit of detection was 0.04 mg·L~(-1),the relative standard deviation values of within-day and between-day analysis were 1.91% and 2.57%,respectively.Two hours after administration,the concentration of methylprednisolone was 1.67 mg·L~(-1) in plasma.CONCLUSION The method is sensitive,accurate,reproducible and can be employed for the determination of the concentration of methylprednisolone in plasma.
【CateGory Index】: R96
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