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《Chinese Journal of Clinical Pharmacy》 2011-05
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Determination of multiple-components in Gandi capsule by HPLC

TANG Yuenian,LING Qidi,XIAO Min,ZHANG Jian,LU Xiaotong(Department of Pharmacy,Xinhua Hospital Affiliated to Medical School,Shanghai Jiaotong University,Shanghai 200092,China)  
AIM To develope a reversed phase high performance liquid chromatography to determine multiple ingredients in Gandi capsule.METHODS Gandi capsule samples were extracted with selective ultrasound extraction.Separation and quantification were achieved by a Ineitsil~(?) ODS-3 column under the optimum conditions.Acetonitrile and 0.5‰phosphoric acid solution(pH 2.25)were used as mobile phase and gradient elution at a flow rate of 1 mL·min~(-1), at room temperature and detected with UV detector at 215 nm.RESULTS The quantified analysis of external standard calibration curves was used.The calibration graph was linear in the range of 0.50-50 g·L~(-1) for astragaloside(AG) and 0.05-50 mg·L~(-1) for baicalin(BCL) with r0.999,respectively.Detection limit for AG was 0.1 g·L~(-1) and for BCL was 0.01 mg·L~(-1).For spiked sample analysis,the RSD was less than 3.5%and the recoveries were 96.89%- 100.00%for AG and BCL.CONCLUSION This method is simple,accurate and convenient,and can be used to determine index components of Gandi capsule.
【Fund】: 上海交通大学医学院科技基金(编号JYY0905)
【CateGory Index】: R286
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