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《Chinese Journal of Clinical Pharmacy》 2012-01
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Effects and mechanisms of GLP-1 on fatty acid metabolism in insulin-resistant 3T3-L1 adipocytes

DONG Yi,YAO Minghui,WANG Yiqun(Department of Pharmacology,Shanghai Medical College,Fudan University,Shanghai 200032,China)  
AIM To explore the effects and mechanisms of glucagon like peptide-1(GLP-1) on fatty acid metabolism.METHODS Through ELISA and Western blot,the present study was to investigate the effects and mechanisms of GLP-1 on fatty acid metabolism in insulin-resistant 3T3-L1 adipocytes which were induced by high glucose and insulin.RESULTS ELISA showed that GLP-1 regulated medium free fatty acid(FFA) concentrations in a insulin dependent way: FFA content rised when GLP-1 combined with insulin;GLP-1 decreased FFA content without insulin.Similarly,GLP-1 increased fatty acid synthase(FAS) content with insulin;when there was no insulin in medium,FAS could not be affected by GLP-1.Western blot revealed that GLP-1 could enhance protein kinase B(PKB) phosphorylation.When treated with PKB phosphorylation inhibitor LY294002 or Wortmannin with insulin could inhibit the increased effect of GLP-1 on FFA content.GLP-1 had no effect on PKB phosphorylation when there was no insulin existence,but could diminish hormone-sensitive lipase(HSL) concentration in this case.CONCLUSION GLP-1 can enhance insulin sensitivity and decrease HSL concentration in insulin resistant 3T3-L1 adipocytes.Insulin affects GLP-1 regulation of fatty acid metabolism in resistance 3T3-L1 adipocytes.
【Fund】: “重大新药创制”科技重大专项(编号2009ZX09303-006);; 国家自然科学基金(编号8100569);; 上海市自然科学基金(编号10ZR1402100);; 上海市重点学科建设项目(编号B119);; 中央高校基本科研业务费专项资金(编号10FX041)
【CateGory Index】: R587.1
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