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Simultaneous content determination of benzoic acid and salicylic acid in a new concentrated benzoic acid and salicylic acid ointment by RP-HPLC

FU Feng~(1,2),SHI Xiaojin~1,GUO Yanping~1,SONG Zhongjuan~2,HUANG Rongfei~2,YU Yunchao~1(1 Department of Pharmacy, Huashan Hospital,Fudan University,Shanghai 200040,China;2 Department of Pharmacy,Huadong Hospital, Fudan University,Shanghai 200040,China)  
AIM To establish a method for the simultaneous content determination of benzoic acid and salicylic acid in a new concentrated benzoic acid and salicylic acid ointment by RP-HPLC.METHODS A Kromasil-C_(18)chromatographic column(150 mm×4.6 mm,5μm)was used;the mobile phase was V(methanol):V(0.02 mol·L~(-1) KH_2PO_4,the pH value was adjusted to 3.1 with H_3PO_4 solution)=50:50;the determination wave length was 280 nm;the flow rate was 1.0 mL'min"1 and the column temperature was 20℃.RESULTS The linear ranges of benzoic acid and salicylic acid were 96-144 mg·L~(_1)(r=0.9995)and 48-72 mg·L~(-1)(r=0.999 7),respectively.Their average recoveries were within 99.6%-100.6%and 99.4%-100.2%.respectively.CONCLUSION The method is simple, accurate,reproducible and suitable for the determination of benzoic acid and salicylic acid levels in a new concentrated benzoic acid and salicylic acid ointment.
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