Simultaneous determination of the content of acetaminophen and sodium benzoate in acetaminophen oral liquid preparation by gas chromatography
LIU Guangqian~1,MO Xuanyong~2,YAO Guoxin~1(1 Department of Pharmacy,Dongguan Tungwah Hospital,Dongguan 523010,China;2 Guoyao Quality Inspection Center in Dongguan City,Dongguan 523010,China)
AIM To establish a gas chromatography method for determining the content of acetaminophen and sodium benzoate content in acetaminophen oral liquid preparation.METHODS Shimadzu GC-17AA gas chromatograph used SE-54 fused silica capillary column(30 m×0.25 mm×0.5μm).The detector was hydrogen flame detector (FID),carrier gas was high purity N_2.The FID temperature was 300℃,injection volume was 1μL,gas split ratio was 20:1,the initial column temperature was 50℃,reached to 120℃with the speed of 25℃·min~(-1)and keeping for 3 min, reached to 170℃with the speed of 8℃·min~(-1)and keeping for 10 min,reached to 250℃with the speed of 10℃·min~(-1) and keeping for 5 min.RESULTS The linear range of acetaminophen and sodium benzoate was 39.87-398.7 mg·L~(-1) (r=0.999 3)and 2.006-6.018 mg·L~(_1)(r=0.999 1),respectively.The regression equation was A=3 552 039ρ+ 57 673,A=450 650ρ+41 921.The average recovery(n=9)was 97.28%(RSD=0.87%)and 95.15%(RSD= 1.32%),respectively.CONCLUSION The method is simple,rapid,specific,and can be used to effectively control the content of acetaminophen and sodium benzoate in acetaminophen oral liquid preparation.
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.