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《China Forestry Science and Technology》 2012-01
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An industrialized technique for raising tissue culture plant of Acacia cincinnata

ZHANG Yue-jiao Test Center of Fujian Forest Science & Technology,Nanjing 363600,Fujian,China  
A research on tissue culture technique of Acacia cincinnata was summarized and the optimal mediums were proposed in this paper.The medium of improved MS+6-BA 0.3mg/L+KT 0.5mg/L+NAA 0.3mg/L+sucrose 30 g/L+carrageenan 8g/L was optimal for inducement,the medium of improved MS+6-BA 0.8mg/L+KT 0.3mg/L+NAA 0.6mg/L+ sucrose 30 g/L+carrageenan 8 g/L was optimal for subculture.The medium of 1/2 improved MS+NAA 0.5mg/L+sucrose 20 g/L+carrageenan 8 g/L was optimal for rooting,and the pH was 5.8.Using these mediums,the inducement rate of industrialized tissue culture of Acacia cincinnata could be up to 70%,effective bud multiplication was more than three times,the rooting rate was more than 95%.
【Fund】: 福建省林业厅种业创新与产业化工程项目(编号:2130106技术推广与培训)
【CateGory Index】: S792.99
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