Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Forestry Engineering》 2018-01
Add to Favorite Get Latest Update

Fabrication and characterization of electro-spun composite fibrous scaffolds for tissue engineering by CNCs-reinforced PCL

WANG Jinru;NI Xiaohui;CHENG Wanli;HAN Guangping;College of Material Science and Engineering,Northeast Forestry University;Key Laboratory of Bio-Based Material Science and Technology,Ministry of Education;  
In order to improve the mechanical properties of polycaprolactone( PCL) fibrous scaffolds,electro-spun pure fibrous scaffolds and composite fibrous scaffolds at various cellulose nanocrystals( CNCs) loading levels were prepared. Obtained by acid hydrolysis,the CNCs were used as a reinforcement. The morphology,crystallinity as well as thermal and mechanical properties of CNCs-filled and unfilled fibrous scaffolds were examined by the scanning electron microscopy( SEM),wide angle X-ray diffraction( WXRD),thermogravimetric analysis( TG),and tensile testing. The influences of the CNCs on the cell compatibility of the PCL fibrous scaffolds were also explored. The SEMimages of the obtained fibrous scaffolds demonstrated that the nanofibers were successfully prepared and the average diameter of the fibers for the scaffolds increased slightly and then decreased with the increased CNCs loading in the fibrous scaffold. The thermal stabilities of the CNCs-reinforced fibrous scaffolds were not higher than electro-spun pure PCL fibrous scaffolds. The crystallinity of the fibrous scaffolds also increased firstly and then decreased with the increase in CNCs loading. Furthermore,the optimal reinforcement conditions of the PCL fibrous scaffolds were obtained,which was 5.25% of the mass ratio of m( CNCs)/m( PCL). The mechanical strength and elongation at break of the PCL/CNCs scaffolds at 5.25% CNCs loading increased by 291% and 320%,respectively,compared with the pure PCL fibrous scaffolds. In addition,pancreatic ductal cancer cells( Panc-1) and hepatic cells( HL7702) derived from human adult were successfully cultured on the PCL/CNCs composite fibrous scaffolds. The results of one-way variance analysis of the proliferation rate for Panc-1 on PCL and CNCs/PCL composite fiber scaffolds indicated that the CNCs did not increase the cytotoxicity of the PCL composite fibrous scaffolds. The CNCs could be used as a reinforcing material to fabricate nanocomposite fibrous scaffolds with good mechanical properties and cytocompatibility through electrospinning,which provided feasible approaches for the application of the CNCs in tissue engineering.
【Fund】: 国家自然科学基金(31470580);; 国家林业局引进先进林业科学技术项目(2013-4-11)
【CateGory Index】: TQ340.64
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved