REUSE OF MEDIUM FOR SUCCESSIVE TRANSFER CULTURE
Dong Yan Zhao Jimei Bie Wanli Hou Yujie (Liaoning Poplar Research Institute Gaizou 115200)
The aim of this study was to cut down cost of tissue culture by reuse of discarded culture medium. By adding 10%,20%,30%,40%,50% culture solution to retreated old medium and with normal culture medium as control, successive transfer culture of triploid of aspen were conducted. The result showed that on old medium added with 30% solution, sprout clump had the similar differentiation and the growth as on the normal medium. It suggests that reused of discarded medium is practicable.
【CateGory Index】： S792.114.04