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《Scientia Silvae Sinicae》 1996-06
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DETECTION OF MYCOPLASMALIKE ORGANISMS(MLO) OF THE PAULOWNIA WITCHES' BROOM TISSUE CULTURE BY A POLYMERASE CHAIN REACTION AMPLIFIING A SEQUENCE OF 16S rDNA

Li Jiangshan; Jin Kaixuan; Wang Yue (The reserch institute of forestry CAF Beijing100091) (The reserch institute of forest protection CAF Beijing100091)Xiong Yaoguo; Qiu Bingsheng(The reserch institute of forestry CAF Beijing100091) (Institute of Microbil  
An oligonucleotide primer set (PaF/PaR) was designed for polymerase chain reaction (PCR) amplification of an approximately 1. 2kb fragment DNA from Paulownia Witches' broom(PaWB) MLO on the basis of 16S rRNA sequence from O-MLO. Amplification of a 1. 2kb DNA fragment from PaWB-MLO-infected plants DNA in total nucleic acid extrats of the host plant Paulownlh spp., but no l. 2kb DNA fragment was observed when healthy DNA was used as a template. The study showed that the PCR method was able todetect PaWB-MLO in as little as 9. 4pg of total DNA from infected Paulernia tissue culture plantlets.
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【CateGory Index】: S763
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