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A Dynamic Observation on Interleukin 1 β Gene Transcription in Neonate Rats with Hypoxic Ischemic Brain Damage

Xiao Fuda,Shi Jinyang,Sun Guilian,et al. Department of Pediatrics,The Second Clinical College,China Medical University,Shenyang 110003  
Objective To study the change of interleukin 1 β gene transcription in the animal model of hypoxic ischemic brain damage(HIBD).Methods A model of HIBD in left brain of neonate rats was established and IL 1 β mRNA in the lesioned ischemic cortex and hippocampus at different times by DNA RNA Dot blot hybridization with IL 1 β oligonucleotide probe was detected.Results Dot blot hybridization was sensitive and specific.The probe can detect 1pg of total RNA,and hybridize only to the homologous nucleic acid.Level of IL 1 β mRNA was elevated as early as 1 hour and peaking expression was 3 hour after HIBD,IL 1 β expression in hippocampus was higher than that in cortex.Conclusions IL 1 β gene expression appears early after HIBD,it may play an important role in the response of inflammatory to HIBD.
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