A NOVEL MOLECULARLY IMPRINTED POLYMER:SYNTHESIS AND ADSORPTION BEHAVIOR OF GATIFLOXACIN

GE Yanru1 MO Chuanjie1 CHEN Zhixian1 YAN Yongsheng2 XU Wanzhen2 College of Pharmacy, Jiangsu University, Zhenjiang 212013, Jiangsu, China College of Chemistry and Chemical Engineering, Jiangsu University Zhenjiang 212013, Jiangsu, China  

A kind of molecularly imprinted polymer (MIPs) with high selectivity was prepared using methacrylic acid (MAA) as functional monomer, ethylene glycol dimethacrylate (EDMA) as crosslinker and Gatifloxacin as template. The effect of various parameters such as volume of solvent, functional monomer dosage, crosslinker dosage and polymerization time were investigated. The selective binding experiment for substrates show that the affinity and selectivity for Gatifloxacin were higher than that for blank polymer. Scatchard analysis show that the MIPs recognized template with two kinds of binding sites. The dissociation constant Kd and maximum adsorption quantity Qmax of these two kinds of binding sites were calculated: Kd1 and Qmax1 of the binding sites with high affinity were 8.67×10-4 mol/L and 28.19μmol/g, while Kd2 and Qmax2 of the binding sites with low affinity were1.05×10-3 mol/L and 33.20μmol/g respectively.

【Key Words】： Gatifloxacin Molecularly imprinted polymer Scatchard analysis Binding sites.
【Fund】： China National Science Foundation (No. 20877036);; Jiangsu University Science Research Funding (No. 04JDG017).
【CateGory Index】： O631.3

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