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《Chinese Journal of Ethnomedicine and Ethnopharmacy》 2009-08
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Determination of Polysaccharides in Astragalus membranaceus with DNS Method

XIA Chun-seng1 WEI Shi-li2 ZHONG Yan-hong1(1.Yangtze River Pharmaceutical Holding Guangzhou Hairui Pharmaceutical CO.,Ltd,Guangzhou 510663,China;2.New Drug Research and Development Center,Guangzhou University of Traditional Chinese Medicine,Guangzhou 510405,China)  
Objective To establish a method for the determination of polysaccharides in Astragalus membranaceus.Method Using optimized DNS colorimetric method to determine the polysaccharides.Result The conditions were as follows:3,5-dinitrosalicylic acid 2.0ml,aqueous bath temperature 100℃,and active time 5min.There was a good liner relationship between the concentration of polysaccharides and the absorbance at the wavelength of 500nm.The average recovery was 100.6%,RSD=1.79%(n=5).Conclusion The method is convenient,accurate with good reproducibility and can be used for the determination of polysaccharides in Astragalus membranaceus.
【CateGory Index】: R284.1
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