Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Ethnomedicine and Ethnopharmacy》 2009-08
Add to Favorite Get Latest Update

Determination of Polysaccharides in Astragalus membranaceus with DNS Method

XIA Chun-seng1 WEI Shi-li2 ZHONG Yan-hong1(1.Yangtze River Pharmaceutical Holding Guangzhou Hairui Pharmaceutical CO.,Ltd,Guangzhou 510663,China;2.New Drug Research and Development Center,Guangzhou University of Traditional Chinese Medicine,Guangzhou 510405,China)  
Objective To establish a method for the determination of polysaccharides in Astragalus membranaceus.Method Using optimized DNS colorimetric method to determine the polysaccharides.Result The conditions were as follows:3,5-dinitrosalicylic acid 2.0ml,aqueous bath temperature 100℃,and active time 5min.There was a good liner relationship between the concentration of polysaccharides and the absorbance at the wavelength of 500nm.The average recovery was 100.6%,RSD=1.79%(n=5).Conclusion The method is convenient,accurate with good reproducibility and can be used for the determination of polysaccharides in Astragalus membranaceus.
【CateGory Index】: R284.1
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 10 Hits
1 LI Wen-chao,LI Jun-long,SHA Fu-sheng,WANG Yong,CHEN Hui-liang(College of Animal Science,Anhui Science and Technology University,Fengyang 233100,China);Comparison of Two Methods in Determination of Polysaccharide Contents of Chrysanthemum morifolium Ramat[J];Journal of Anhui Science and Technology University;2010-06
2 Li Ruiyan,Gao Jianping Department of Pharmacology,Changzhi Medical College;The Study to Method of Content Determination of Radix Codonopsis Water-soluble Polysaccharides[J];Journal of Changzhi Medical College;2010-04
3 DANG Juna,b WANG Yinga,b TAO Yan-Duoa SHAO Yuna MEI Li-Juana WANG Qi-Lanaa(Northwest Institute of Plateau Biology Chinese Academy of Sciences,Xining 810008,P.R.China)b(Graduate University of Chinese Academy of Sciences,Beijing 100049,P.R.China);Determination of Polysaccharide in Armillarialuteo-rivens Mycelium Extract[J];Chinese Journal of Spectroscopy Laboratory;2011-06
4 Han Wei Huang Xi Zhang Lingling Lü Jiankun Qiu Shaobing (Engineering Center for Traditional Chinese Medicine Modernization, East China University of science and technology, Shanghai 200237,China);Advances in the Extraction,Isolation,Purification and Assay Methods of Polysaccharides in Chinese Traditional Medicine[J];Mechanical and Electrical Information;2012-14
5 ZHENG Lu;LEI Ming-ke;ZHANG Rui;WU Yuan-xin;State Key Laboratory of Agricultural Microbiology,Huazhong Agricultural University;School of Chemical Engineering and Pharmacy,Wuhan Institute of Technology;;Studies on Immobilizing α-amylase with Sodium Alginate[J];Hubei Agricultural Sciences;2013-23
6 JIA Liang-liang,YUAN Ding*,HE Yu-min,ZHANG Chang-cheng(Medical College of China Three Gorges University,Yichang 443002,Hubei,China);The Development of Extraction,Separation and Assay of Polysaccharides[J];Food Research and Development;2011-03
7 YANG Hao,ZHANG Yao-wu,ZHANG Long(Department of Pharmacy,college of Chemical Engineering,Qingdao University of Science and Technology,Qingdao Shandong 266042,China);Determination of polysaccharides in epimedium with DNS Method[J];Tianjin Chemical Industry;2012-01
8 ZHAO Qi-duo1,SHU Le-xin1,MA Lin1,CUI Guo-hong2(1.Tianjin University of Traditional Chinese Medicine,Tianjin 300193 China; 2.Changchun Terumo medical appliance Limited,Changchun 130012 China);Determination of polysaccharide in Huaiganjunzhi by phenol-sulfuric acid method[J];Journal of Yichun College;2011-08
9 YIN Ming-hua,CAO Zhen-long(College of Life Sciences,Shangrao Normal University,Shangrao 334001,Jiangxi China);The Effects of KT and NAA on Proliferation of Astragalus membranceus Stem with a Bud and its Induction of Callus and Rooting[J];Subtropical Plant Science;2011-01
10 ZHANG Yan-li1,Abdurahman·Holly2,Aytulun·Simayil1(1.Pharmacy College,Xinjiang Medical University,Urumqi 830011,China;2.Xinjiang Uygur Autonomous Region’s Uygur Hospital,Urumqi 830000,China);Study on content determination of Kunlun Chrysanthemum polysaccharides from Uygur medicine by phenol-sulfuric acid method[J];Chinese Journal of Pharmaceutical Analysis;2010-11
【Citations】
Chinese Journal Full-text Database 4 Hits
1 Zhang Xinxu Gao Haibo (No.5 19 Pilot Plant Trial Office,Scicntific Rcscarch Center, Guizhou Chitianhua Group Co.,Ltd.,Chishui Guizhou,564707);Determination of Polyose in Chinese Guni Caterpillar Fungus by Spectrophotometry[J];Guizhou Chemical Industry;2002-03
2 CHEN Qiying,SUN Xueqi,XU Xiaoxia Sichuan Provincial Institute for Drug Control,Chengdu 610036 China;QUANTITATIVE DETERMINATION OF POLYSACCHARIDE IN ARMILLARISIN ORAL LIQUID BY 3,5-DINITROSALICYLIC ACID COLORIMETRY[J];WEST CHINA JOURNAL OF PHARMACEUTCAL SCIENCES;2000-03
3 WANG Hong-ying1,QIAN Srguleng1,ZHAO Qian-cheng2,ZANG Shu1,JIN Feng-xie1(1.College of Bio.& Food Technology,Dalian Institute of Light Industry,Liaoning Dalian 116034,China;2.Department of Food Technology,Dalian Fisheries University,Liaoning Dalian 116023,China);Determination of Polysaccharide in Ophiopogon japonicus with 3,5-Dinitrosalicylic Acid Colorimeitry[J];Journal of Shenyang Agricultural University;2005-05
4 YANG Li1, WANG Zhi-Hua2, TAO Jian-Sheng2 (1. Dept. of Pharmacy, Ruijin Hospital Affiliated to Shanghai Jiaotong University, Shanghai 200025; 2. College of Pharmacy, Shanghai University of Traditional Chinese Medicines, Shanghai 201203);Comparison of the Methods for Determination of Astraglus Polysaccharides in Radix Astragali[J];Chinese Journal of Pharmaceuticals;2005-09
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 XU Guo(Chaohu Product Quality Supervision and Inspection,Chaohu 238000,China);Determination of Content of Paeoniflorin in Radix Paeoniae Alba by HPLC[J];Anhui Chemical Industry;2012-02
2 Liu Chong1 et al.(1 Institute of Agricultural Sciences in Coastal Area of Jiangsu Province,Yancheng 224002,China);Progress of Study on Energy Plant Euphorbia lathyris. L.[J];Anhui Agricultural Science Bulletin;2011-23
3 YANG Gui-ming et al(Institute of Sericulture,Chengde Medical College,Chengde,Hebei 067000);Study on the Determination Factor of Reduced Sugar with DNS Spectrophotometry[J];Journal of Anhui Agricultural Sciences;2006-14
4 MENG Li et al (Henan Institute of Science and Technology,Xinxiang,Henan 453003);Effect of the Maceration Extraction from the Vegetables Umbelliferae Family on Pleurotus ferulae Lanzi Hypha Growth[J];Journal of Anhui Agricultural Sciences;2006-19
5 DONG Yan-li et al(Traditional Chinese Veterinary Medicine College,Hebei Agricultural University,Dingzhou,Hebei 073000);The Effect of Ultramicro-Pulverization on the Astraglus Polysaccharides Dissolubility[J];Journal of Anhui Agricultural Sciences;2008-09
6 KANG Jing et al (Department of Life Sciences,Xinxiang Medical College,Xinxiang,Henan 45003);Isolation and Identification of Fucoidanase Freshwater Bacterium[J];Journal of Anhui Agricultural Sciences;2009-13
7 CAO Yuan-xiang et al(Life Sciences and Engineering Department,Southwest Jiaotong University,Chengdu,Sichuan 610031);Research Advance of Quality Control Techniques of Ophiopogon japonicus[J];Journal of Anhui Agricultural Sciences;2009-33
8 LUO Hong-bin et al (Medical School of Hubei University for Nationalities,Enshi,Hubei 445000);Analysis of nrDNA-ITS Gene Sequence of Famous-Region Drug Codonopsis tangshen Oliv.[J];Journal of Anhui Agricultural Sciences;2010-09
9 WANG De-fu et al(Guizhou Institute for Drug Control,Guiyang,Guizhou 550004);HPLC Determination of Berberine Hydrochloride in Shihu Yeguang Pills[J];Journal of Anhui Agricultural Sciences;2010-20
10 XIA Wei-fu et al(College of Life Science,Hunan University of Arts and Science;Changde,Hunan 415000);Some Commonly Used Herbs and Identification of Counterfeit Goods[J];Journal of Anhui Agricultural Sciences;2010-33
China Proceedings of conference Full-text Database 9 Hits
1 ZHUANG Jin-qiu1,2,MEI Jian-guo1,2,SHEN Zhi-qiang1(1.Shandong Binzhou Animal Science & Veterinary Medicine Academy,Binzhou 256600,China;2.College of Animal Science and Veterinary Science,Jilin University,Changchun 130062,China);BIOLOGICAL FUNCTION OF ASTAGALUS POLYSACCHARIDE AND ITS PROGRESS IN STUDY AND APPLICATION IN SWINE PRODUCTION[A];[C];2012
2 Wei Bing,Xu Min,Yu Lei,YANG Yun(College of Pharmacy,Henan University of Traditional Chinese Medicine,Zhengzhou 450008.China);Isolation,Purification and Structural Analysis of Oligosaccharide from Rhizoma polygonati[A];[C];2011
3 Lin Xianzhe, Huang Xuesong* (Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China);Determination of Piperine in the Flower,Young and Ripen Fruit of Pepper[A];[C];2009
4 ;蒙成药嘎日迪-5对小鼠和大鼠行为、镇痛作用的影响[A];[C];2008
5 TIAN Lian-qi,SHI Yan-bang,ZHANG Ben-shan,ZHANG Zhen-ling,LI Bo (Henan College of Chinese Medicine,Zhengzhou,Henan,450008,China );The Study and Comparison of Ultraviolet Spectrum Group of Medicinal Semen Pharbitidis(Heichou and Baichou)Decoction Pieces[A];[C];2009
6 WANG Jie1,GUO Li-li1,ZHOU Ting-ting2,CHEN Wen1 Cardiovascular Disease-Syndrome Pattern Research Laboratory,Guang’anmen Hospital,China Academy of Traditional Chinese Medicine,Beijing 100053,China Life science and Biopharmaceutical College,Shenyang Pharmaceutical University,Shengyang 110016,China;Mechanism and Compatibility of Traditional Sedatives and Tranquilizers Chinese Medicine in Treatment of Cardiovascular Disease[A];[C];2010
7 BAO Xiao-hua1,Bagenna1,BAI Ming-gang1 (1.College of Mongolian Medicine and Pharmacy,Inner University for Nationalities,Tongliao 028000,China);Origin and Advance for MONGOLIAN Medicine Xieriga Decoction of Four Drugs[A];[C];2010
8 LI Sheng-he,JIN Guang-ming,WANG Jue,XU Wan-xiang,ZHOU Jin-xing,DING Xue-zhen (Animal Science College,Anhui Science and Technology University,Fengyang 233100);Effects of Astragalus Polysaccharide on Mice Male Genital Organs of Sleep Deprivatio[A];[C];2010
9 Xiangde Zhao 1,2(1.Zhengzhou University College of Pharmacy,Zhengzhou 450001;2.Institute of Medical Science of Henan Province,Zhengzhou 450052);Study of quality standards for Cai Huang Granules[A];[C];2012
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 LIU Xi-hui et al (Guangxi Key Laboratory of Crop Genetic Improvement and Biotechnology,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007);Extraction and Content Determination of Polysaccharide in Sugarcane Leaves[J];Journal of Anhui Agricultural Sciences;2007-34
2 LIAN Jia-sheng et al (Department of Horticulture,College of Agronomy,Yanbian University,Longjing,Jilin 133400);Preliminary Study on the Tissue Culture of Adventitious Root in Astragalus membrcmaceus[J];Journal of Anhui Agricultural Sciences;2008-07
3 YANG Yan-jie et al (Luohe Medical College,Luohe,Henan 462002);Studies on Anti-oxidant and Liver-protective Effects of the Extracts from the Root of Kiwifruit[J];Journal of Anhui Agricultural Sciences;2008-19
4 WANG Fu-ke (Physiology Department of the Basic Science School in Ningxia Medical College,Yinchuan,Ningxia 750004);Research on the α-Amylase Immobilization[J];Journal of Anhui Agricultural Sciences;2008-21
5 ZHAO Xiu-juan et al (Biological and Chemical Engineering School,Inner Mongolia University of Science and Technology,Baotou,Inner Mongolia 014010);Study on Tissue Culture and Radiation Mutation of Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao[J];Journal of Anhui Agricultural Sciences;2009-09
6 HUANG Sheng-quan et al(College of Light Industry and Food Sciences,South China University of Technology,Guangzhou,Guangdong 510640);Study on Extraction Technology of Water-soluble Polysaccharide from Ganoderma lucidum by Ultrasonic Assistant Method[J];Journal of Anhui Agricultural Sciences;2009-23
7 GONG Ru-chun et al(Department of Biology,Tonghua Normal University,Tonghua,Jilin 134002);Effect of Actinidia arguta(Sieb. et Zucc.) Planch. on Intestinal Muscle of Rabbit[J];Journal of Anhui Agricultural Sciences;2011-04
8 LUO Deng-hong et al(Department of Biology,Huanggang Vocational & Technical College,Huanggang,Hubei 438002);Hypoglycemic Activity of(RHIZOMA NELUMBINIS NUCIFERAE) Polysaccharide and Its Effect on In Vivo Antioxidant Activity[J];Journal of Anhui Agricultural Sciences;2011-06
9 WEI Tao et al(College of Arts & Science,Beijing Union University,Beijing 100191);Hypoglycemic Activity of Polysaccharides Extracted from Tremella Aurantialba Mycelium in Experimental Diabetic Mice[J];Journal of Anhui Agricultural Sciences;2012-12
10 LI Xin-xin et al(College of Biological and Agricultural Engineering,Jilin University,Changchun,Jilin 130000);Research and Application Progress of Edible Film[J];Journal of Anhui Agricultural Sciences;2012-22
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Yan-li1,RU Xianguli2,DING Hai-yan1,WANG Yan1,Aytulun·Simayil1(1 College of Pharmacy,Xinjiang Medical University,Xinjiang Urumqi 830011; 2 Hospital of Chinese Medicine,Xinjiang Urumqi 830011,China);Studying on Ultrasonic-Microwave Synergistic Extraction Flavonoids of Kunlun Chrysanthemum[J];Guangzhou Chemical Industry;2011-21
2 PAN Ying1, LI Ning1, NI Hui2, MENG Da-li1, JIA Xiao-guang2 1. Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China 2. Xinjiang Institute of Chinese Materia Medica and Ethical Materia Medica, Urumqi 830002, China;Advances in research on chemical constituents in plants of Coreopsis L. and their pharmacological activities[J];Drugs & Clinic;2012-05
3 YE Ju1,LIN Hai-ming1*,CHENG Wei-dong2,MA Min3,QIU Dai-yu1 (1.College of Agronomy,Gansu Agricultural University,Lanzhou,Gansu Province 730070,China; 2.School of Traditional Chinese Medicine,Southern Medical University,Guangzhou,Guangdong Province 510515,China; 3.College of Chemistry and Life Science,Qinghai University for Nationalities,Xining,Qinghai Province 810007,China);Growth and Slope Effects for Yield and Quality of Hedysarum polybotrys Hand.-Mazz.[J];Acta Agrestia Sinica;2013-02
4 SUN Li-li,DONG Yin-mao,LI Li (Chinese Cosmetics Research Center,Beijing Technology and Business University,Beijing 100048);Study on Extraction and Its Efficacy of Polysaccharides from Sprouting Mungbean[J];Northern Horticulture;2013-09
5 Abuduhalili·aili,Xilizhati·abulaiti,Guhainisa·maihemuti,Halidan·abulimiti,Pataer·keyimu,Ayitulun·simayi(School of Pharmacy,Xinjiang Medical University,Xinjiang Urumqi 830011,China);Study on Ultrasonic-microwave Synergistic Extraction of Total Flavonoids from Chrysanthemum[J];Guangzhou Chemical Industry;2013-07
6 DAI Yuanchen,HOU Hongman,ZHANG Gongliang(School of Food Science and Technology,Dalian Polytechnic University,Dalian 116034,China);Enrichment medium design and growth kinetics research of Lactobacillus bulgaricus[J];Journal of Dalian Polytechnic University;2013-04
7 HOU Wu-Ai;YUE Zhi-Jin;College of Chemistry and Chemical Engineering,Shanxi Datong University;;Determination of Polysaccharide from Astragalus Mushroom by Phenol-Sulfuric Acid Method[J];Chinese Journal of Spectroscopy Laboratory;2013-05
8 JIANG Bao-ping;XU Li-jia;JIA Xiao-guang;XIAO Pei-gen;Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College;Xinjiang Institute of Chinese Medica and Ethnodrug;;Research progress on the chemical constituents and pharmacological activities of Coreopsis tinctoria[J];Drugs & Clinic;2014-05
9 DING Yao-guang;MA Fu-hua;GOU Ru-hu;Department of Chemistry and Life Science,Gansu Normal University for Nationalities;;Study on the extraction process and content determination of polysaccharides in Potentilla anserina[J];Guangdong Agricultural Sciences;2014-11
10 WANG Chun-xia;GAO Peng;WANG Xiao-mei;Qingdao Bright Moon Sea Science and Technology Co. Ltd;;Processing Technology of Sargassum Extract[J];Journal of Anhui Agricultural Sciences;2014-31
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Zhong Guogan, Jiang Yan, Wei Yongdi, An Zhanyuan,Sun Xiaoxia,Li Yunyi (Depart of Physiology, Central Laboratory of Physiology, Depart of Organic Chemistry);Positive inotropic action of astragalus membranaceus atponins on lsolated Working Heart[J];JOURNAL OF NORMAN BETHUNE UNIVERSITY OF MEDICAL SCIENCE;1994-05
2 SUN Yan, YANG Jie Department of Pharmaceutics, General Hospita l of PLA, Beijing 100853, China;Experimental study of the effect of Astragalus membranaceus against herpes simplex virus type1[J];Journal of First Military Medical University;2004-01
3 Zhang Hongbo Harbin Pharmaceutical Group Medicine Co.,Ltd.(150010);Study on Astraglus Leaves Extract Method and Determination of Astraglus Polysacchride Content[J];Heilongjiang Medical Journal;2005-01
4 CHEN Qiying,SUN Xueqi,XU Xiaoxia Sichuan Provincial Institute for Drug Control,Chengdu 610036 China;QUANTITATIVE DETERMINATION OF POLYSACCHARIDE IN ARMILLARISIN ORAL LIQUID BY 3,5-DINITROSALICYLIC ACID COLORIMETRY[J];WEST CHINA JOURNAL OF PHARMACEUTCAL SCIENCES;2000-03
5 Zhu Qizhong Sun Xun Sun Baoju Yue Hui (Biology Department of Heze Teachers College, Heze, Shandong 274015);Comparative Study of the Absorption Spectrometry of Reducing Sugar with DNS Reagent[J];Journal of Heze Teachers College;1999-02
6 Wu Yong 1,Ou Yang Jingping 2,Tu Shuzhen 2,Wang Changhua 2 1.Hubei College of TCM.Wuhan 430061 2.Medical College,Wuhan University Wuhan 430071;Effect of Astragalus Polysaccharide on Pathologic Change of Micrangium of Diabetic Rats and Experimental Study on the Mechanism[J];Jounal of Hubei College of Trditional Chinese Medicine;2001-03
7 LI Zhijun , WEI Lianbo, HE Feng, et alNan Fang hospital, Guangzhou (510515);Effect of Astragalus polysacchride on the Mesangial proliferative glomerulonephritis in rat's[J];Chinese Journal of Integrated Traditional and Western Nephrology;2000-04
8 Jiang Qinglin; et al;Effects of Asl on insulin and C-peptide[J];HEILONGJIANG MEDICINE AND PHARMACY;1999-03
9 Ren Yuhao,Hu Yuanliang,Liu Jiaguo,Zhang Baokang and Song Dalu (College of Veterinary Medicine,Nanjing Agric Univ,Nanjing 210095,China);The effects of astragalus polysaccharide, epimedium polysaccharide and epimedium flavone on cell infecting of NDV[J];Journal of Nanjing Agricultural University;2001-02
10 HUANG Ping 1, WU Qinghe 1, XU Honghua 1, CHEN Wenliang 2, RONG Xianglu 1, DENG Xiangchao 1, HAN Jian 1 (1.Guangzhou University of TCM,Guangzhou 510405,China;2.Shenzhen Pharmaceutical Factory of Chinese Materia Medica,Shenzhen 518028,China);Effects of Compound Lingzhi Jiangtang Capsule on Experimental Diabetes[J];JOURNAL OF GUANGZHOU UNIVERSITY OF TRADITIONAL CHINESE MEDICINE;2000-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved