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《Chinese Journal of Ethnomedicine and Ethnopharmacy》 2009-10
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Screening Specific Expressed Genes of Breast Cancer by SSH

WANG Yan ZGHONGShijun SHAO Min ZHANG Qingfeng(ZUNYi Medical College School of Zhu Hai,GUANG Dong,Zhu Hai,519041)  
Objective In order to study the molecular mechanism of breast cancer,to screen the genes differentially expressed in breast cancer.Methods Suppression subtractive hybridization(SSH)combine reverse dot blotting was performed to isolate the differentially expressed genes between breast cancer and normal mammary tissues.A subtractive cDNA library of highly expressed genes in breast tumor tissue was established.Result About 22 differentially expressed genes were obtained.By sequencing and BLAST analysis we found 2 genes are new genes.Conclusion SSH is an effective method with high specificity to detect specific expressed genes in breast cancer genesis and development.
【CateGory Index】: R737.9
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【References】
Chinese Journal Full-text Database 1 Hits
1 ZHOU Jian1,YUAN Chuantao2,DAI Gong1(1.Department of Pharmacology,Weifang Medical College,Weifang 261053,China;2.Department of Pathology,the Affiliated Hospital of Jining Medical College,Jining 272000,China);The research of JNKK1 expression and the effect on the metastasis and prognosis of breast carcinoma[J];China Medical Herald;2010-16
【Citations】
Chinese Journal Full-text Database 1 Hits
1 WU Xiao jiang, CHEN Ke neng, XUE Guang wei (Department of Surgery, School Oncology, Peking University, Beijing 100036, China);A novel marker and therapeutic target of breast cancer HER2 and trastuzumab[J];China Oncology;2002-02
【Co-citations】
Chinese Journal Full-text Database 4 Hits
1 TAO Jin-zhong1,2,ZHANG Yong2,ZHAO Xing-xu2,et al(1.School of Agriculture,Ningxia University,Yinchuan 750021,China;2.College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China);Differential screening and identification mastitis resistant genes of dairy cows in mammary tissue by the reverse Northern dot-blotting[J];Heilongjiang Animal Science and Veterinary Medicine;2008-11
2 HUANG Feng-lan,Hu Guo-fu,HU Bao-zhong College of Life Sciences,Northeast Agriculture University,Harbin150030,China;The application of suppression subtractive hybridization in gene cloning[J];Letters in Biotechnology;2004-04
3 CAO Sui-zhong 1,2 , LI Hong-bin 2,WANG Ai-hua 2, ZHAO Xing-xu 1, DU Li-xin 2* (1. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China; 2. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China);Construction of Mastitis Resistant cDNA Library in Dairy Cows Using Suppression Subtractive Hybridization[J];Chinese Journal of Animal and Veterinary Sciences;2005-06
4 ZHANG Yu-Wei;LAI Mao-De Zhejiang University, School of Medicine, Department of Pathology, Hangzhou 310006, China;A Non-radioactive Method Used For Reverse Northern[J];Hereditas(Beijing);2004-03
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