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《Chinese Journal of Ethnomedicine and Ethnopharmacy》 2009-10
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Screening Specific Expressed Genes of Breast Cancer by SSH

WANG Yan ZGHONGShijun SHAO Min ZHANG Qingfeng(ZUNYi Medical College School of Zhu Hai,GUANG Dong,Zhu Hai,519041)  
Objective In order to study the molecular mechanism of breast cancer,to screen the genes differentially expressed in breast cancer.Methods Suppression subtractive hybridization(SSH)combine reverse dot blotting was performed to isolate the differentially expressed genes between breast cancer and normal mammary tissues.A subtractive cDNA library of highly expressed genes in breast tumor tissue was established.Result About 22 differentially expressed genes were obtained.By sequencing and BLAST analysis we found 2 genes are new genes.Conclusion SSH is an effective method with high specificity to detect specific expressed genes in breast cancer genesis and development.
【CateGory Index】: R737.9
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