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《Chinese Journal of Ethnomedicine and Ethnopharmacy》 2009-17
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Determination of Hydrochloride Berberine in Milinqing Tablets by HPLC

LIU Yuan 1 SONG Zhi-zhao 1 MO Hai-tao2Wen zhi-yun1LI Xing-yu1 1.Guangxi Institute for Chinese Medicine & Pharmaceutical Science,Guangxi Province,530022;2.Guanfeng Pharmaceutical Co.Ltd.of Nanning,Guangxi,530008)  
OBJECYIVE: Aim to set up determination the contents of Hydrochloride Berberine in Milinqing Tablets.METHODS:HPLC method was used.Chromatographic condition included Hypersil ODS2(C18,4.6×250mm,5μm) and the mobile phase consisting of acetonitrile-0.05mol/l triethylamine solution(29:71),with a detection wavelength of 345nm and the temperature to room temperature.RESULT:The calibration curves were linear in the range of 10.2~61.2μg/mL for Hydrochloride Berberine,r=0.9993,average recovery of Hydrochloride Berberine Was 97.5%,RSD Was 0.34%(n=5).CONCLUSIONS: This method is simple,accurate,reproducible and canbe used as the determination of the preparations.
【CateGory Index】: R286
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