Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Farm Products Processing》 2015-18
Add to Favorite Get Latest Update

Development of a Duplex PCR Method for Detecting Proteolytic Pseudomonas

NIU Chunhua;ZHAO Yujuan;GAO Lei;Duan Cuicui;LI Shengyu;Institute of Agro- Food Technology, Jilin Academy of Agricultural Sciences,National R&D Center for Milk Processing;  
Pseudomonas is a kind of key psychrophilic bacteria affecting the quality of raw milk. This study aimed to establish a duplex PCR method to detect the pseudomonas and thermophilic protease. Several pairs of specific primers are designed for 16 S r RNA gene and apr X gene of Pseudomonas according to the gene sequences from Gen Bank, and PCR method is used to screen proper primers. Two pairs of selected primers are used to optimize the reaction conditions of duplex PCR. Meanwhile,this method is used to detect the Pseudomonas and common intestinal pathogenic bacterium. The results show that only the 16 S r DNA and apr X genes from Pseudomonas with proteolytic activity could be amplified. It can be concluded that the duplex PCR is specific and could be used in detecting proteolytic Pseudomonas.
【Fund】: 吉林省世行贷款农产品质量安全项目(2011-Y35);; 现代农业产业技术体系专项资金资助(CARS-37)
【CateGory Index】: TS252.1
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LI Long-zhu,LI Wen-feng,MA Ting-ting,ZHANG Fu-xin * ,SU Wei-li,ZHAO Jian-jun,WU Su, AI Dui,CHANG Ying,LIU Ling (College of Food Engineering and Nutritional Science of Shaanxi Normal University,Xi’an 710062,China);Different storage temperature influencing the raw material feta microbiological quality[J];Science and Technology of Food Industry;2013-12
2 ZHAO Li-jun;XIE Jing;FENG Shu-jiao;QIAO Li-jun;College of Food Science and Technology,Shanghai Ocean University;;Isolation and identification of proteolytic spoilage bacteria in chilled pork[J];Science and Technology of Food Industry;2014-07
3 YU Yan-yan;DING Tian;LIU Dong-hong;College of Biosystems Engineering and Food Science,Zhejiang University;;Advancements on rapid detection methods for psychrophilic bacteria in raw milk[J];Science and Technology of Food Industry;2014-11
4 NIU Pei Hua;ZHANG Chen;WANG Ji;TAN Wen Jie;MA Xue Jun;Key Laboratory for Medical Virology,Ministry of Health, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention;;Detection and Identification of Six Foodborne Bacteria by Two-tube Multiplex Real Time PCR and Melting Curve Analysis[J];生物医学与环境科学(英文版);2014-10
5 SHI Pujie;LI Lin;LIU Meng;WANG Yun;TU Maolin;YANG Jian;FAN Fengjiao;DU Ming;ZHANG Lanwei;School of Food Science and Engineering,Harbin Institute of Technology;;Isolation and Identification of Thermostable Lipase Produced by Pseudomonasfluorescens from Fresh Milk[J];Journal of Dairy Science and Technology;2015-01
6 XU Yu;REN Jing;State Key Laboratory of Dairy Biotechnology,Bright Dairy Research Institute, Bright Dairy and Food Co. Ltd.;;Progress in Research on Main Psychrophilic Bacteria with Emphasis on Psychrophilic Bacillus in Raw Milk[J];Journal of Dairy Science and Technology;2015-02
7 XU Yu;REN Jing;State Key Laboratory of Dairy Biotechnology,Research Center of Bright Dairy & Food Co.Ltd.;;Research progress in hazards and control of psychrotrophilic Pseudomonas in raw milk[J];Science and Technology of Food Industry;2015-13
8 WANG Jiao;ZHANG Yun;LIU Shu-ming;LIU Wen-li;East university of Heilongjiang;Heilongjiang Beingmate Dairy Co.,Ltd.;;Hazard Analysis and Control of Psychrotrophic Bacterias and Heat Resistant Enzymes in Raw Milk and Dairy Products[J];Food Research and Development;2015-13
9 GAO Lin;CHEN Wan-yi;REN Jing;State Key Laboratory of Dairy Biotechnology,Dairy Research Institute,Bright Dairy & Food Co.,Ltd.;College of Food Science & Technolgy,Shanghai Ocean University;;Research progress of hazards and rapid methods for detection of Pseudomonas Fluorescen in foods[J];Science and Technology of Food Industry;2015-16
10 WANG Wei-jun;LI Yan-hua;Beingmate Baby & Child Food Co.,Ltd.;College of Food Science and Biotechnology,Zhe Jiang Gong Shang University;;Comparing Detection Methods for Extracellular Thermostable Enzymes Secreted by Psychrophile in Milk[J];Food Research and Development;2015-15
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Shao~1,LIU Gui-hua~2,QIAO Feng~1,SHI Jie-ping~1,FANG Li~3,LI Bo~ 1* (1. Department of Epidemiology,School of Public Health,Jilin University,Changchun 130021,China; 2. Center for Disease Prevention and Control of Jilin Province,Changchun 130012,China;3. Health and Epidemic Prevention Station,Jilin Petroleum Combine Limited-Liability Corporation ,Songyuan 138000,China);Establishment of PCR method for detection of Staphylococcus aureus[J];Journal of Jilin University(Medicine Edition);2006-05
2 Zhao Chuanpeng~1 Pu Yuepu~1 Yin Lihong~1 Liang Geyu~1 Lü Xiwu~2 Li Xianning~2(~1School of Public Health,Southeast University,Nanjing 210009,China)(~2College of Civil Engineering,Southeast University,Nanjing 210096,China);Detection of abundance of Pseudomonas in environmental samples by real-time quantitative PCR[J];Journal of Southeast University(Natural Science Edition);2006-01
3 WANG Ya~(1,2),WANG Zhe~(1),XU Chuang~(1),DENG Jun-liang~(2),YANG Feng-lin~(2)(1.College of Animal Science and Veterinary Medicine,Jilin University,Changchun,Jilin,130062,China;2.College of Animal Science and Technology,Sichuan Agriculture University,Ya'an,Sichuan,625014,China);Progress on Methodology for the Quantific of Nucleic Acids[J];Progress in Veterinary Medicine;2006-07
4 Wei Ling, Yang Huanghao, Chen Xiaolan, Qu Huiying, Xu Jingou * (Department of Chemistry, The Key Laboratory of Analytical Sciences of Ministry of Education, Xiamen University, Xiamen 361005);Determination of Nucleic Acids Based on Shifting the Association Equilibrium between Tetrasulphonated Aluminum Phthalocyanine and Alcian Blue[J];Chinese Journal of Analytieal Chemistry;2002-08
5 Zheng Jingsheng 1,2 Lü Bei 1 1 Hainan Provincial Key Laboratory of Crop Molecular Breeding,Sanya,572025 2 The Institute of Rice&wheat,Fujian Academy of Agricaltural Sciences,Fuzhou,350019;PCR Technique and its Practical Methods[J];Molecular Plant Breeding;2003-03
6 Guo Xinzhu et al;The application of PCR in food detection[J];Guangzhou Food Science and Technology;2001-02
7 ZHANG Wen-chao~(1,2*),LIU Xiao-guang~1,WU Qin-qin~1 (1. College of Information Science and Engineering ECUST, Shanghai 200237, China; 2. College of Information Engineering, Liaoning University of Petroleum & Chemical Technology, Fushun 113001,China);Research and Application of Temperature Control System of Gene Amplifing and Analyzing (PCR) Instrument[J];Journal of East China University of Science and Technology;2004-02
8 Li Congzhi Tan Deming Lu Menghou Wu Ying Zhou Wenhong Liu Guozhen Liu Anguo (Department of Infection, Xianya Hospital, Hunan Medical University, Changsha 410008);PCR for the detection of 16S rRNA gene bacteria[J];BULLETIN OF HUNAN MEDICAL UNIVERSITY;1999-02
9 Yang Xiangyu, Song Jian, Feng Rongxiu * (College of Pharmaleuticals & Biotechnology,Tianjin University,Tianjin 300072,China);Advancement of Study in the Fluorescent Label Dyes[J];Chemistry;2003-09
10 Nie Libo1, Zhang Liming2, Hou Qinglin1, He Quanguo 1, (1Hunan Key Laboratory of Green-Packaging and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou 412008; 2Chemistry and Chemical Engineering College, Sun Yat-sen University, Guangzhou 510275);Applications of Optical Methods in DNA Detection[J];Chemistry;2007-08
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved