A HUMAN×MOUSE HYBRIDOMA IMMORTALIZED IN VITRO
Mao Yiping Shan Xiangnian Lu Xiaoxuan Wang Shijun
A hybridoma from mouse myeloma cell line SP2/0(HGPRT-) and human peripheral lymphocytes.was obtained in the presence of PEG and selected in hypoxanthine-aminopterine-thymidine (HAT) medium.It was proved that 5-12 human chromosomes were retained in the hybrid population,with no interspecific chromosome translocation observed.Monocloned A4 population retained human chromosomes 9、11、X、14、18、19 and 22.The electrophoretic zymogram of G6PD in hybridoma shows a three-banded pattern, suggesting both parents specific activities were expressed.This sort of human × mouse hybridoma could be used in human gene mapping,human immunoglobulin chain gene cloning and human monoclonal antibody producing.
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| Chinese Journal Full-text Database |
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