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《Journal of Nanjing Railway Medical College》 1985-01
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Mao Yiping Shan Xiangnian Lu Xiaoxuan Wang Shijun  
A hybridoma from mouse myeloma cell line SP2/0(HGPRT-) and human peripheral lymphocytes.was obtained in the presence of PEG and selected in hypoxanthine-aminopterine-thymidine (HAT) medium.It was proved that 5-12 human chromosomes were retained in the hybrid population,with no interspecific chromosome translocation observed.Monocloned A4 population retained human chromosomes 9、11、X、14、18、19 and 22.The electrophoretic zymogram of G6PD in hybridoma shows a three-banded pattern, suggesting both parents specific activities were expressed.This sort of human × mouse hybridoma could be used in human gene mapping,human immunoglobulin chain gene cloning and human monoclonal antibody producing.
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Chinese Journal Full-text Database 7 Hits
1 Feng Jialin;Shao Songsheng;Lin Yaping;Liu Hongzhen;Zou Meijun Shanghai Institute of Radiation Medicine, Shanghai;DOSE RESPONSE CURVE FOR HUMAN BLOOD LYMPHOCYTE CHROMOSOME ABERRATIONS INDUCED BY ~(60)CO γ RADIATION OF LOW DOSES USING PREMATURE CONDENSED CHROMOSOME(PCC)[J];Journal of Radiation Research and Radiation Processing;1990-02
2 Zhou Deyi;Su Dan;Zhang Shanping Agriculture School of Wugang, Hunan;INVESTIGATION OF THE MAGNETIC FIELD INDUCING HUMAN RED BLOOD CELL FUSION[J];Journal of Natural Science of Hunan Normal University;1987-03
3 Pan Yongchen;Niu Fuwen Department of Histology and Embryology;Ihe studyo of the factors influencing cell fusion[J];;1989-03
4 Li Baolian, Liang De-bai, Liu Yu, Dong Hongyan, Zou Yuhong, Xue Shepu (Department of Cell Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences);THE TECHNIQUES OF CELL FUSION OF SINGLE PARENT ANUCLEATING CELL[J];;1984-04
5 Ou Xiaoming, Wang Junming, Huang Chen, et al (Department of Biology);FUSION OF HEPATOMA CELL[J];Journal of Xi'an Jiaotong University(Medical Sciences);1992-01
6 Mao Yiping Lu Xiaoxuan Shan Xiangnian Wang Shijun (Department of Biology,Nanjing Railway Medical College);A Human-Mouse Hybridoma Immortalized in vitro[J];Acta Genetica Sinica;1986-01
7 Yan Yongshan;Influence of pH in Fusion System on Plating Efficiency of Hybrid Cells[J];Hereditas(Beijing);1983-04
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