CROWTH SUPPRESSION OF FIVE CANCER CELLS BY ADENOVIRUS-MEDIATED WTP53 GENE
Shi Guoli, Feng Yuxin, Liu Cong, Zhao Liqing, Fang Xin, Chen Defeng (Institute of Molecular Biology, Nankai University, Tianjin, 300071)
A recombinant adenovirus (AdCMVp53) was constructed. Five human cancer cells PG (lung carcinoma), CAE (colon carcinoma), HCT (colon carcinoma), HeLa (cervix carcinoma) and BEL7402 (liver carcinoma) were infected with AdCMVp53, and the cell growth inhibitory effects of AdCMVp53 were determined by counting cell number. According to IC 50 (doses required to kill 50% of cells) and the cell growth curves, dose dependent death induced by wtp53 was observed in the cells infected by AdCMVp53 in different cell lines. Three cell lines, i.e. PG, CAE and BEL7402, of which IC 50 were below 15MOI (multiplicity of infection), were more sensitive to doses of AdCMVp53 than HeLa and HCT cell, of which IC 50 were 100MOI and 80MOI respectively. The results showed a possible therapeutic strategy in PG (lung cancer), CAE (colon carcinoma) and BEL7402 (hepatoma).