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《Ningxia Medical Journal》 2018-08
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Establishment of cardiac dysfunction model after cardiopulmonary resuscitation in rats with cardiac arrest

LIU Min;QI Wenjuan;WANG Feng;WANG Xiaohong;Ningxia Medical University;Intensive Care Unit,General Hospital of Ningxia Medical University;Brain Laboratory,Ningxia Medical University;  
Objective To establish the cardiac dysfunction model after cardiopulmonary resuscitation in rats with cardiac arrest.Methods The CA-CPR model was established by intravenous injection potassium chloride(4 ℃) combined with asphyxiation for 4 min and then followed by artificial chest compression for 3 min. Thirty male SD rats were divided into two groups: sham-operated group(with relevant catheter only,no CPR,n = 15) and CPR group(with inserted related catheters and being performed CPR,n = 15).Mean arterial pressure(MBP),the maximal derivatives of left ventricular pressure(dp/dtmax),and the maximal rate of pressure decline(-dp/dt) were monitored continuously for 4 hrs after resuscitation(catheterization). The ultrastructure of rat myocardial tissue was observed by transmission electron microscope. Serum samples were taken 4 hrs after resuscitation(catheterization),and lactate dehydrogenase(LDH) activity was measured by lactate-pyruvate method. Serum creatine kinase isoenzyme(CK-MB) concentration was measured by ELISA. Results In CPR group,The MBP,dp/dtmax,and-dp/dt were significantly lower at different time points after resuscitation than those before CPR(P 0. 05); MBP,dp/dtmax,-dp/dt in CPR group were significantly lower than those in sham operation group at each time point after resuscitation(P 0. 05); Compared with sham-operated group,ultrastructural damage in myocardial was significant in CPR group; Compared with sham-operated group,LDH activity and CK-MB concentration in CPR group were significantly higher(P 0. 05). Conclusion The model of cardiac dysfunction after cardiopulmonary resuscitation(CA-CPR) in rats are successfully established,and the model is stable and reliable,and to provide a good model basis for exploring protection measures of post-resuscitation cardiac dysfunction.
【Fund】: 国家自然科学基金资助项目(81460288);; 宁夏自然科学基金资助项目(NZ1242)
【CateGory Index】: R541.78;R-332
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