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《Ningxia Medical Journal》 2018-08
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The establishment of an animal model of osteolysis induced by titanium particles in murine calvaria

CHEN Desheng;LI Yan;GUO Fengying;ZHAO Jiangbo;TIAN Jianing;XIA Gen;ZHU Xi;The General Hospital of Ningxia Medical University;Ningxia Medical University;  
Objective To establish the osteolysis animal models induced by titanium particles in murine calvaria.Methods 30 male BALB/c mice were randomly divided into control group and model group,each group was only 15. The mouse calvaria was cut open and the periosteum was exposed. 0. 3 mL titanium particles mixture was injected into the periosteum defect area of rat in the model group and 0. 3 mL sterile fluid of PBS was injected in the control group. The surface of the murine calvaria of the periosteum defect area was observed by macroscopic observation,pathological and tartrate-resisitant acid phosphatase staining examination after fourteen days. Results In macroscopic observation,the surface of the murine calvaria of periosteum defect area was holonomic and had bright luster in the control group. In the model group,the surface of the murine calvaria of periosteum defect area was attacked by titanium particles and bone absorption. The pathologic results showed that the bone absorption was not significant,the bone trabecula was evenly distributed,arranged regularly,thick and dense,and no titanium particles have been found in the control group,however,bone absorption was obvious,cell morphology was irregular,bone trabecula was sparsely disturbed,spacing widened and bone trabecula interruption phenomenon was appeared in the model group. The bone resorption area was(0. 098 ± 0. 012) mm2 in the control group,and(0. 365 ± 0. 113) mm2 in the model group,the two groups had significant differences. The TRAP staining results showed that the staining was shallow,the positive staining area was smaller,and the number of cells was not large in the control group and the staining was deep,the positive staining area was larger,the number of cells was increased significantly and there was bone absorption phenomenon in the model group. The number of TRAP-positive cells were(11. 42 ± 1. 21) in the control group and(27. 82 ± 0. 24) in the model group,there was a significant difference between the two groups. Conclusion Using the titanium particles to induce osteogenesis in a mouse skull is simple,economical and reproducible method. It is a recommendable experimental method for further study of aseptic loosening.
【Fund】: 国家自然科学基金项目(81560364);国家自然科学基金项目(81760405);国家自然科学基金项目(81760395);; 宁夏人事厅留学资助基金项目(2017659)
【CateGory Index】: R687.4;R-332
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