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《Ningxia Medical Journal》 2018-08
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Effects of lycium barbarum polysaccharide on the apoptosis of human ovarian cancer cell line HO-8910PM and its mechanism

GUO Hua;DONG Hui;LV Juan;ZI Shanshan;ZHANG Xu;YANG Zhijuan;Department of Gynecology,General Hospital of Ningxia Medical University;Scientific Research Equipment Management Center,General Hospital of Ningxia Medical University;Beijing Biochip National Engineering Research Center,General Hospital of Ningxia Medical University;  
Objective To investigate the effect of lycium barbarum polysaccharide as a reversal agent on the proliferation and apoptosis of human ovarian epithelial carcinoma cell line HO8910 PM and its possible mechanism. Methods Half inhibition rate of LBP and PTX single drug action cells was detected by CCK8 method. 25% and 50% of inhibition rate concentration were selected as experimental concentration. Cell cycle and apoptosis were detected by flow cytometry. The expressions of HO8910 PM cells Survivin,caspase-3,Bax,bcl-2 mRNA and protein were detected by RT-PCR and western blot after the synergistic effects of LBP and PTX. Results After synergistic treatment,LBP synergistic PTX action on HO8910 cells showed that apoptosis rate was greater than the sum of single drug action,and the gain effect was significant(P 0. 05). LBP in cooperation with PTX in HO8910 cells showed that the proportion of cells at G0/GI stage and G2/M stage increased significantly,and the cell cycle inhibition was significantly enhanced. LBP PTX action of HO8910 cells mediated by Survivin,BCL-2 gene mRNA DE protein expression was significantly reduced(P 0. 05),Caspase 3,Bax gene mRNA and protein expression significantly increased(P 0. 05),and presented the concentration dependence. Conclusion LBP can be used as a synergistic sensitizing strong paclitaxel in the anti-cancer ability of HO8910 PM cells,and its mechanism may be related to the down-regulation of Survivin,bcl-2 gene expression and the up-regulation of caspase-3 and Bax gene expression.
【Fund】: 宁夏自然科学基金资助项目(NZ14158)
【CateGory Index】: R285.5
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