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Teratogenic Effects of Chlorpyrifos on Embryos of Microhyla pulchra

LIU Ying;PAN Bo;HU Yan;JIANG Lei;LIN Yong;Institute of Environment and Plant Protection,Academy of Tropical Agriculture Sciences of China;Danzhou Scientific Observing and Experimental Station of Agro-Environment,Ministry of Agriculture,P.R.China;  
[Aims] The teratogenic effects of chlorpyrifos on the embryos of Microhyla pulchra were evaluated.[Methods] FETAX assay was adopted to study the teratogenic effects of chlorpyrifos on the embryos of M. pulchra.[Results] The results showed that chlorpyrifos can induce malformations of embryos, including axial flexures, skin hypopigmentation and cephalic dysplasia. The total malformation rate and the percentage of three kinds of abnormalities increased with the increase of exposure dose of chlorpyrifos, it presents a dose-effect relation. The LC_(50)(96 h) of chlorpyrifos to embryos of M. pulchra was 1.97 mg a.i./L, the EC_(50)(96 h) was 1.00 mg a.i./L, and the teratogenic index(TI) value was 1.97. [Conclusions] The chlorpyrifos has a potential teratogenic risk to the embryos of M. pulchra.
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