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《Chinese Journal of Clinical Rational Drug Use》 2017-35
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Analysis on SSR information in Radix Tetrastigmae transcriptome

HUANG Qiao-wen;LIU Hong-xu;DENG Si-shan;Department of Pharmacy,People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine;  
Objective Through the analysis of the transcriptome data of Erastigma hemsleyanum Diels ET,developing Radix Tetrastigmae SSR molecular marker technology and provide methods for genetic diversity analysis and resource conservation.Methods The unigene obtained from the high-throughput sequencing of Radix Tetrastigmae was excised and analyzed by Simple Sequence Repeat(SSR).Results Within the 84 433 unigene,8 263 eligible SSR were searched,with a frequency of 9.79% SSR loci per 11.72 kb on average.There are a total of 174 kinds of repeat motifs here,and the number of tetranucleotide repeat motifs is up to 68.The proportion of(A/T) n,(T/A) n,(GA/TC) n constitute a higher percentage.Four,five,six nucleotide has lower frequency accounting for 1.61%.The length of SSR was concentrated within 10~24 bp,of which there were 1 053 10 bp constituting largest amount,followed by 1 042 of 15 bp.The length of 11,12,14,18 bp and more than 500,which were 689,970,576,895 respectively.There are 4 880 Radix Tetrastigmae with length at 12 to 20 bp,accounting for59.02%,which was theoretically moderate above polymorphism.Conclusion The analysis and mining of the SSR information of the Radix Tetrastigmae transcriptase sequence provided a reference for the genetic diversity analysis,molecular breeding,molecular marker development and genetic resources protection of Radix Tetrastigmae.
【Fund】: 福建省科技厅自然科学基金项目(No:2016J01351);; 福建省属公益类科研所基本科研专项(No:2015R1031-3)
【CateGory Index】: S567.239
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