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STUDIES ON DIGESTIVE ENZYMES OF MARINE MOLLUSC Ⅰ. PRELIMINARY ASSAY AND APPLICATIONS OF THE DIGESTIVE ENZYMES OF MYTILUS EDULIS LINNE CHARYBDIS JAPONICA AND LITTORINA SP.

Liu Wanshun LiPin(Shandong College of Oceanology) (Science Publishing House, Academia Sinica)  
We determined the enzymes activities of the digestive system in Mytilus edulis、 Charybdis japonica and Littorina with 11 enzymes substrates, and studied preliminarily the applications of these digestive enzymes mixtures.Application experiments of the digestive enzymes showed thatPlatycephalus egg fertilization membrane can be removed with 1.0% digestive enzymes of Mytilus edulis at 25℃ in 20 minutes(see Fig 3). A large amount of protoplasts of Saecharomyces Cervisiae can be prepared with 1.0% digestive enzymes of Mytilus edulis (with equal volume of 0. 5% semicellulase and pectinase added) at 30℃ in 60 minutes(see Fig 9), and most of the cell walls of Phaeodactylum tricornutum can be removed in 1.5-2 hours under the same conditions (see Fig 5).We determined the enzymes activities of the digestive system in Mytilus edulis、 Charybdis japonica and Littorina with 11 enzymes substrates, and studied preliminarily the applications of these digestive enzymes mixtures.Application experiments of the digestive enzymes showed thatPlatycephalus egg fertilization membrane can be removed with 1.0% digestive enzymes of Mytilus edulis at 25℃ in 20 minutes(see Fig 3). A large amount of protoplasts of Saecharomyces Cervisiae can be prepared with 1.0% digestive enzymes of Mytilus edulis (with equal volume of 0. 5% semicellulase and pectinase added) at 30℃ in 60 minutes(see Fig 9), and most of the cell walls of Phaeodactylum tricornutum can be removed in 1.5-2 hours under the same conditions (see Fig 5).
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